Effects of Methylene Blue on Ovarian Torsion?Detorsion Injury in a Rat Model - 24/09/20
, Ismet Hortu, MD, PhD-c 2, Gokay Ozceltik, MD 2, Gurkan Yigitturk, PhD 3, Oytun Erbas, MD 4, Nedim Karadadas, MD 2Abstract |
Study Objectives: |
Methylene blue (MB) is an antioxidant that ameliorates ischemia-reperfusion injury in several tissues. We analyzed the effects of MB as an inhibitor of torsion–detorsion injury in rat ovaries.
Methods |
Rats were randomly divided into 5 groups. Group 1 was the sham group, in which only laparotomy was performed. Group 2 was the torsion group, with 3 hours of ischemia. Group 3 was the torsion + MB group, with 3 hours of ischemia after MB administration. Group 4 was the torsion–detorsion group, with 3 hours of ischemia and reperfusion. Finally, group 5 was the torsion–detorsion + MB group, with 3 hours of ischemia and MB administration before detorsion/reperfusion. Ovary injuries were histopathologically scored. Malondialdehyde (MDA) and total protein levels in ovarian tissues were determined, and long pentraxin-3 (PTX3) levels were measured in ovarian tissue using an enzyme-linked immunosorbent assay.
Results |
In comparing group 4 with group 5 and group 2 with 3, histopathological parameters reflecting injury were significantly increased in groups 4 and 2. Group 3 generated increased MDA levels when compared with group 2 (P < .05). However, there was no significant difference between groups 2 and 3 in terms of plasma PTX3 levels. MDA and PTX3 levels decreased in group 5 in comparison with group 4 for MDA (P < .000) and PTX3 levels (P < .01).
Conclusions |
MB alleviated ischemia-reperfusion ovary injury in our experimental model.
Le texte complet de cet article est disponible en PDF.Key Words : Ovarian torsion, Perfusion-reperfusion injury, Methylene blue, Ovarian detorsion, Ischemic injury, Rat model
Plan
| All authors declare no conflict of interest. |
|
| Authors' Contributions: Authors' contributions are as follows: IH: design of the study; GO, OE: data collection; OE, OI, GY: procedures; GY, GO: data analysis; OI: manuscript writing; IH, OI, NK: revision, editing, rewriting. |
Vol 33 - N° 5
P. 506-510 - octobre 2020 Retour au numéro
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