Four point-of-care lateral flow immunoassays for diagnosis of COVID-19 and for assessing dynamics of antibody responses to SARS-CoV-2 - 06/08/20
, Po-Ren Hsueh b, d, ⁎ , Shyr-Chyr Chen a
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Highlights |
• | Performance of serological tests detecting SARS-CoV-2 antibodies is disease course dependent. |
• | Detection sensitivity of rapid antibody tests with lateral flow immunoassay for COVID-19 reached 100% after 3 weeks of symptom onset. |
• | COVID-19 patients with pneumonia exhibited earlier seroconversion than those without pneumonia. |
• | Prolonged viral shedding after seroconversion with a median duration of 14 days was observed. |
• | Serological testing may be a useful tool in addition to rRT-PCR for the diagnosis of COVID-19. |
SUMMARY |
Objectives: |
We aimed to evaluate the role of rapid serological tests in the management of coronavirus disease 2019 (COVID-19) patients.
Methods: |
This retrospective study enrolled 16 real-time reverse transcription polymerase chain reaction-confirmed symptomatic patients with COVID-19 and 58 COVID-19 negative patients at a medical center in Taiwan over a 3-month period. Serial serum samples were collected and tested for antibody response using four point-of-care (POC) lateral flow immunoassays (LFIA) (ALLTEST 2019-nCoV IgG/IgM Rapid Test, Dynamiker 2019-nCoV IgG/IgM Rapid Test, ASK COVID-19 IgG/IgM Rapid Test, and Wondfo SARS-CoV-2 Antibody Test). Time-dependent detection sensitivity and timeliness of seroconversion were determined and compared between the four POC rapid tests.
Results: |
The overall sensitivity and specificity of the four tests for detecting anti-SARS-CoV-2 antibodies after 3 weeks of symptom onset were 100% and 100%, respectively. There was no significant difference between the rapid tests used for detection of IgM and IgG separately and those used for detection of combined total antibody (mainly IgM/IgG). There was no significant difference between the four POC rapid tests in terms of time required for determining seroconversion of COVID-19. Patients with COVID-19 with pneumonia demonstrated shorter seroconversion time than those without pneumonia.
Conclusion: |
Though the POC antibody rapid tests based on LFIA showed reliable performance in the detection of SARS-CoV-2-specific antibodies, the results of these tests should be interpreted and applied appropriately in the context of antibody dynamic of COVID-19 infection. COVID-19 patients complicated with pneumonia exhibited earlier anti-SARS-CoV-2 antibody response than COVID-19 patients without pneumonia.
Le texte complet de cet article est disponible en PDF.Keywords : COVID-19 pandemic, Antibody response, Rapid test, Immunochromatographic assay, Lateral flow method
Plan
Vol 81 - N° 3
P. 435-442 - septembre 2020 Retour au numéro
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