Phylogenomic assessment of drug-resistant Mycobacterium tuberculosis strains from Beira, Mozambique - 28/07/20
, Anzaan Dippenaar b , Emilyn Costa Conceição c , Cinara Feliciano a , Margarida Maria Passeri do Nascimento a , Kamila Chagas Peronni d , Wilson Araújo Silva d, e , Josefo João Ferro f , Lee H. Harrison g , Robin Mark Warren b , Valdes Roberto Bollela a, ⁎ Abstract |
Background |
Mozambique is a high-burden tuberculosis (TB) country where TB/HIV co-infection and drug resistant TB (DR-TB) incidence is increasing. Whole genome sequencing (WGS) comprehensively describes the molecular epidemiology of TB, allows prediction of DR-TB phenotypes, lineages strains identification and better understanding of transmission chains.
Objective |
To describe genetic diversity of DR-TB Mycobacterium tuberculosis isolated in Beira, Mozambique.
Methods |
Descriptive cross-sectional study with 35 M. tuberculosis isolates, resistant to at least one first-line drug on molecular drug-susceptibility tests (DST). Variant identification, DR prediction and phylogenetic analysis provided by WGS, drug-susceptibility pattern compared to line-probe assay (LPA): Genotype MTBDRTMplus and MTBDRTMsl.
Findings |
Lineage 4 (L4) was the most prevalent: 25 (71.4%) isolates; 5 (14.3%) L1 and 5 (14.3%) L2. WGS showed 33/35 (94.3%) isolates resistant to at least one drug, two pan-susceptible isolates that were previously diagnosed as DR-TB with genotype MTBDRplus. Concordance between WGS and LPA: 88.6% for isoniazid (INH), 85.7% to rifampicin (RPM), 91.4% for quinolones and 100% to second line injectable drugs. There were three possible TB transmission chains, 10 strains showing recent transmission.
Conclusion |
WGS provided reliable information about the most frequent lineages related to DR-TB in Beira, Mozambique: L4.3 (LAM), L2 (Beijing) and L1 (EAI) and possible recent transmission chain.
Le texte complet de cet article est disponible en PDF.Keywords : Mycobacterium tuberculosis, Drug-resistant, Whole genome sequencing, Phylogeny, Mozambique
Plan
Vol 121
Article 101905- mars 2020 Retour au numéro
Article précédent
- The Many Hosts of Mycobacteria 8 (MHM8): A conference report
- Michelle H. Larsen, Karen Lacourciere, Tina M. Parker, Alison Kraigsley, Jacqueline M. Achkar, Linda B. Adams, Kathryn M. Dupnik, Luanne Hall-Stoodley, Travis Hartman, Carly Kanipe, Sherry L. Kurtz, Michele A. Miller, Liliana C.M. Salvador, John S. Spencer, Richard T. Robinson
- Drug repurposing: Fusidic acid as a potential inhibitor of M. tuberculosis FtsZ polymerization – Insight from DFT calculations, molecular docking and molecular dynamics simulations
- Olayinka I. Akinpelu, Monsurat M. Lawal, Hezekiel M. Kumalo, Ndumiso N. Mhlongo
Bienvenue sur EM-consulte, la référence des professionnels de santé.
L’accès au texte intégral de cet article nécessite un abonnement.
Déjà abonné à cette revue ?