IL-32? suppressed atopic dermatitis through inhibition of miR-205 expression via inactivation of nuclear factor-kappa B - 22/07/20
, Jin Tae Hong, PhD a, ⁎ Abstract |
Background |
IL-32 is a novel cytokine involved in many inflammatory diseases. However, the role of IL-32γ, an isotype of IL-32, in atopic dermatitis (AD) has not been reported.
Objective |
We investigated the effects of IL-32γ on development of AD and its action mechanisms.
Methods |
We used phthalic anhydride (PA) and an MC903-induced AD model using wild-type and IL-32γ transgenic mice. We conducted the therapy experiments by using recombinant IL-32γ protein in a reconstructed human skin model and PA-induced model. We conducted a receiver operating characteristic analysis of IL-32γ with new AD biomarkers, IL-31 and IL-33, in serum from patients with AD.
Results |
Dermatitis severity and epidermal thickness were significantly reduced in PA- and MC903-induced IL-32γ transgenic mice compared with in wild-type mice. The concentration of AD-related cytokines was reduced in PA- and MC903-induced IL-32γ transgenic mice compared with in wild-type mice. Subsequent analysis showed that IL-32γ inhibits miR-205 expression in PA- and MC903-induced skin tissue samples and TNF-α/IFN-γ–treated HaCaT cells. IL-32γ reduced NF-κB activity in skin tissue samples from PA- and MC903-induced mice and TNF-α/IFN-γ–treated HaCaT cells. NF-κB inhibitor treatment with IL-32γ expression further suppressed expression of inflammatory mediators as well as miR-205 in TNF-α/IFN-γ–treated HaCaT cells. Furthermore, recombinant IL-32γ protein alleviated AD-like inflammation in in vivo and reconstructed human skin models. Spearman correlation analysis showed that serum levels of IL-32γ and miR-205 were significantly concordant in patients with AD.
Conclusion |
Our results indicate that IL-32γ reduces AD through the inhibition of miR-205 expression via inactivation of NF-κB.
Le texte complet de cet article est disponible en PDF.Key words : Atopic dermatitis, IL-32γ, miR-205, NF-κB
Abbreviations used : AD, AUC, BAY, IκBα, ILC2, miRNA, NF-κB, PA, RHS, ROC, Treg, TSLP, WT
Plan
| Supported by a National Research Foundation of Korea (Republic of Korea) grant funded by the Korean government (grants MRC 2017R1A5A2015541 and 2018R1A2B2001225). |
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| Disclosure of potential conflict of interest: The authors declare that they have no relevant conflicts of interest. |
Vol 146 - N° 1
P. 156-168 - juillet 2020 Retour au numéro
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