Inferior vena cava stenosis-induced deep vein thrombosis is influenced by multiple factors in rats - 18/06/20
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Highlights |
• | Male and female rats weighing 220−260 g are more suitable for establishing a model of IVC stenosis-induced DVT. |
• | IVCs stenosed with silk and monofilament sutures exhibited no difference in leukocyte influxes. |
• | Side branch ligation is not recommended in IVC stenosis-induced DVT in rats. |
Abstract |
Background |
The pathogenesis of deep vein thrombosis (DVT) is incompletely understood, requiring reliable animal models. Inferior vena cava (IVC) stenosis model mimics human DVT.
Objective |
To provide optimal conditions for establishing a rat model of IVC stenosis-induced DVT.
Methods |
Effects of suture, and body weight, sex and side branches of rats on the IVC stenosis model were evaluated. 1 d after modeling, the weight and length of thrombosed IVCs and side branch distance were measured. Histopathological change and leukocytes influxes were observed by hematoxylin and eosin staining. Ly-6G-positive neutrophils were located by immunofluorescence. A multiple regression linear model was then built.
Results |
IVCs stenosed with silk or monofilament sutures presented no difference in leukocyte influxes. Thrombus of 220−340 g rats was significantly heavier than that of 180−220 g rats. Although no statistic difference was found in thrombus weight between male and female rats weighing 180−260 g, males weighing 260−300 g formed larger thrombi than weight-matched females. Thrombus weight and length of rats except 180−220 g females was not impacted by side branch ligation and side branch distance. The regression model showed that sex and body weight were key factors affecting thrombus weight.
Conclusions |
Male and female rats weighing 220−260 g are more suitable for establishing a model of DVT induced by stenosing IVC with silk and without side branch ligation.
Le texte complet de cet article est disponible en PDF.Abbreviations : DAPI, DVT, HPFs, IVC, O.C.T. Compound, SD
Keywords : Deep vein thrombosis, Rat model, Body weight, Sex, Side branch
Plan
Vol 128
Article 110270- août 2020 Retour au numéro
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