Evaluation of the synergetic effect of Yupingfeng san and Flos Sophorae Immaturus based on free radical scavenging capacity - 18/06/20
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Graphical abstract |
Highlights |
• | Decocting YPS and FSI together can better extract flavonoids and polysaccharides. |
• | DPPH assay confirmed that MYP had the synergistic antioxidant effect in vitro. |
• | Measuring the level of oxidation factors can determine the antioxidant ability of MYP. |
• | MYP had a better free radical scavenging effect in vivo than YPS. |
Abstract |
Objective |
This study aimed to determine the optimal extraction process and examine whether the combination of Flos Sophorae Immaturus (FSI) and Yupingfeng san (YPS) has a synergistic effect on free radical scavenging capacity.
Design and methods |
The time of immersion and extraction and the ratios (material/solvent) of the combination of YPS and FSI were optimized on the basis of polysaccharide and flavonoid yields via orthogonal design. The optimal result was used in the 1,1-diphenyl-1-picrylhydrazyl (DPPH) assay and animal experiments to test the antioxidant activity, which is reflected by superoxide dismutase, malondialdehyde, glutathione peroxidase, and total antioxidant capacity serum levels. The optimal extraction process was determined using various ingredients to obtain complex extracts with high active ingredient content and antioxidant activity. DPPH assay results showed that the optimized ingredients have antioxidant effects, and the combination had better antioxidation function than YPS in vitro. The combination also showed synergistic antioxidant activity compared with YPS in vivo.
Conclusions |
The combination of YPS and FSI had a synergistic antioxidant effect in vitro. The optimized extracts had antioxidant effects in vivo. These results indicated that YPS could be used with FSI to improve its antioxidant capacity in the body on the basis of free radical scavenging capacity.
Le texte complet de cet article est disponible en PDF.Abbreviations : TCM, S. japonica, FSI, A. mongholicus, A. macrocephala, S. divaricata, MYP, YPS, DPPH, T-AOC, GSH-Px, SOD, MDA, ROS
Keywords : Yupingfeng san, Flos Sophorae Immaturus, Free radical scavenging capacity, Antioxidant, Optimum extraction process
Plan
Vol 128
Article 110265- août 2020 Retour au numéro
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