Topical co-administration of Teucrium polium hydroethanolic extract and Aloe vera gel triggered wound healing by accelerating cell proliferation in diabetic mouse model - 30/05/20
pages | 12 |
Iconographies | 10 |
Vidéos | 0 |
Autres | 0 |
Graphical abstract |
Co-administration of TPEO and AVGO accelerated excision wound healing in diabetic wound model by (Ⅰ) amplifying antioxidant status (Ⅱ) reducing inflammatory reactions inflammation (Ⅲ) up-regulating growth factors VEGF, FGF-2, IGF-1 and GLUT-1 expressions and (Ⅵ) enhancing endothelial cells, fibroblasts, as well as keratinocytes proliferation.
Highlights |
• | 1-TPEO + AVGO co-administration decreased Proinflammatory markers and inflammatory phase. |
• | 2- TPEO+AVGO co-administration raised mRNA expression of VEGF, IGF-1, GLUT-1 and FGF-2. |
• | 3- TPEO+AVGO co-administration increased tissue antioxidant status. |
Abstract |
Diabetic wounds are major issues in patients with diabetes. Medicinal plants of Teucrium polium and Aloe vera have antioxidant and anti-inflammatory properties that may be profitable for diabetic patients. This study was conducted to evaluate the effect of co-administration of ointments prepared from Teucrium polium hydroethanolic extract (TPEO) and Aloe vera gel (AVGO) on excisional wound healing in a diabetic mouse model. Following the induction of diabetes and circular excisional wound (7 mm), the mice were divided into six groups, namely (Ⅰ) control mice treated with mupirocin (as a standard drug), (Ⅱ and Ⅲ) the mice treated with 5 and 10 % TPEO, (Ⅳ and Ⅴ) the mice treated with 5 and 10 % AVGO, and (Ⅵ) the mice treated with a combination of 5% TPEO and 5% AVGO (TPEO+AVGO). To investigate the wound area, we further evaluated the wound area ratio, histological analysis and the serum levels of tissue antioxidant capacity (TAC) and malondialdehyde (MDA), tumor necrosis factor-α (TNF-α), and interleukin-1β (IL-1β), immunohistochemistry staining for vascular endothelial growth factor (VEGF), insulin-like growth factor (IGF-1), glucose transporter-1(GLUT-1) and collagen type 1 and mRNA expression levels for VEGF, IGF-1, GLUT-1 and fibroblast growth factor-2 (FGF-2). The results showed that administration of the ointments, especially in combination form, shortened the inflammatory phase and reduced the levels of tissue MDA, TNF-α and IL-1β compared to mupirocin group (P < 0.05). Moreover, fibroblasts proliferation, collagen deposition, VEGF, IGF-1, GLUT-1-positive cells and level of TAC, and expressions of VEGF, IGF-1, GLUT-1 and FGF-2 were significantly (P < 0.05) increased in TPEO and AVGO, and especially in the mice treated with the mixed form. Therefore, topical co-administration of TPEO + AVGO accelerated open diabetic wound healing through shortening the inflammatory phase and increasing cell proliferation and collagen deposition.
Le texte complet de cet article est disponible en PDF.Abbreviations : AVGO, FGF, IGF-1, IL-1β, GLUT-1, MDA, TAC, TNF-α, TPEO, VEGF
Keywords : Diabetic wound healing, Teucrium polium, Aloe vera, Glucose transporter type 1, Antioxidant status
Plan
Vol 127
Article 110189- juillet 2020 Retour au numéroBienvenue sur EM-consulte, la référence des professionnels de santé.
L’accès au texte intégral de cet article nécessite un abonnement.
Bienvenue sur EM-consulte, la référence des professionnels de santé.
L’achat d’article à l’unité est indisponible à l’heure actuelle.
Déjà abonné à cette revue ?