Curcumin regulates the differentiation of naïve CD4+T cells and activates IL-10 immune modulation against acute lung injury in mice - 14/03/20
, Fang Xu a, ⁎ Bienvenue sur EM-consulte, la référence des professionnels de santé.
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| pages | 11 |
| Iconographies | 7 |
| Vidéos | 0 |
| Autres | 0 |
Graphical abstract |
Highlights |
• | Curcumin suppressed inflammatory mediators, alleviated lung injury and increased CD4+CD25+Foxp3+ Tregs in ALI model. |
• | Curcumin can upregulate the differentiation from CD4+ naïve T cells to Tregs in vitro. |
• | Curcumin can promote Tregs ratio and increase Tregs derived IL-10 immune modulation. |
• | Curcumin down-regulates lung M1 macrophages and up-regulates M2 macrophages in ALI. |
Abstract |
Objectives |
Acute lung injury/acute respiratory distress syndrome (ALI/ARDS) is one type of respiratory failure characterized by rapid onset of widespread inflammation in the lungs. Curcumin has been reported to be an anti-inflammatory factor through enhancing the function of regulatory T cells (Tregs). This study aimed to explore the effect of curcumin on the differentiation of Tregs and the role of curcumin in ALI/ARDS.
Methods |
A cecal ligation and puncture (CLP)-induced acute lung injury mouse model was used to explore the effect of curcumin in ALI/ARDS. The severity of lung injury was evaluated. Immunohistochemistry of IL-17A and MPO in lung tissue was examined. Treg-related cytokine levels in serum and bronchoalveolar lavage fluid (BALF) were tested. The expression of nuclear factor-kappa B (NF-κB) in lung tissue was detected. Macrophages in lung tissue were detected by immunofluorescence. Splenic CD4+CD25+FOXP3+ Tregs were quantified, and the differentiation of Tregs from naïve CD4 + T cell and STAT5 was evaluated. The expression of IL-10 during naïve CD4 + T cell differentiation in vitro was tested.
Results |
Curcumin alleviated lung injury in the induced CLP mouse model and suppressed inflammation. IL-17A, MPO-producing neutrophils, and NF-κB p65 expression in lungs of CLP mice decreased significantly after pretreatment with curcumin. We found curcumin could regulate M1/M2 macrophage levels in lungs of CLP mice. This may have been through regulating the differentiation of Tregs and the production of Treg-derived IL-10. Treg-derived IL-10 is the main factor that could affect macrophage polarization. We found curcumin could increase Treg proportions in vivo and up-regulate IL-10 expression in serum and BALF of CLP mice. In our in vitro experiments, we found curcumin could promote Treg differentiation and increase the production of IL-10.
Conclusions |
Curcumin can reduce the degree of severity of ALI and uncontrolled inflammation through promoting the differentiation of naïve CD4 + T cells to CD4+ CD25+ FOXP3+ Tregs. Curcumin promotes the conversion of macrophages from M1 to M2. The differentiation of Tregs induced by curcumin may be one source of IL-10 immune modulation.
Le texte complet de cet article est disponible en PDF.Keywords : Curcumin, Acute respiratory distress syndrome, CD4+T cell differentiation, IL-10
Plan
Vol 125
Article 109946- mai 2020 Retour au numéro
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