Green tea and black tea inhibit proliferation and migration of HepG2 cells via the PI3K/Akt and MMPs signalling pathway - 14/03/20
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Graphical abstract |
Highlights |
• | This research used black tea and green tea, which were processed from the same species of fresh leaves by different processing techniques. |
• | Both black tea and green tea showed the apoptosis in HepG2 cells. Green tea was stronger than black tea in cell apoptosis induction. |
• | Black tea and green tea induced cell apoptosis through PI3K/Akt, and inhibited cell migration and invasion through MMPs signaling pathway. |
Abstract |
Background and aims |
Black tea and green tea were produced via different processing techniques from the same tea leave variety. Then, biochemical components of the two water extracts were analysed to study cell apoptosis, migration and invasion of HepG2 cells induced by black tea and green tea.
Method |
The monomer components of the black tea and green tea extracts were analysed by colorimetry and HPLC, with MTT assay and colony formation assays used to assess cell proliferation and viability. The effects of black tea and green tea on apoptosis of HepG2 cells were verified by flow cytometry, with wound healing and Transwell experiments used to detect cell invasion and metastasis. The expression of PI3K/Akt signalling and apoptosis-related proteins as well as epithelial-mesenchymal transition (EMT) regulatory factor in HepG2 cells were determined by western blotting after black tea and green tea treatment.
Results and conclusions |
Black tea and green tea extracts demonstrated different degrees of inhibition of cell migration and invasion, with green tea inducing more HepG2 cell apoptosis. In addition, green tea and black tea extracts inhibited the growth of HepG2 cells and induced apoptosis via PI3K/Akt, and inhibited cell migration and invasion through the MMPs signalling pathway. This study revealed the effects of fermented (black tea) and non-fermented tea (green tea) on liver cancer cells, providing a basis for the investigation of tea extracts for their anti-tumour potential.
Le texte complet de cet article est disponible en PDF.Abbreviations : CG, EGCG, C, EGC, EC, GC, GCG, ECG, GA, TP, AA, TFs, TRs, TBs, MTT, PI3K, AKT, Bax, Bcl-2, MMP-2/9
Keywords : Tea, HepG2, Tumour proliferation, Tumour migration, PI3K/Akt pathway
Plan
Vol 125
Article 109893- mai 2020 Retour au numéro
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