Aberrant DNA methylation of PAX1, SOX1 and ZNF582 genes as potential biomarkers for esophageal squamous cell carcinoma - 13/11/19
, Guo Wang a, b, c, d, ⁎ Bienvenue sur EM-consulte, la référence des professionnels de santé.
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Graphical abstract |
Pyrosequencing is considered the gold standard for methylation detection. Using pyrosequencing to detect the DNA methylation statue of PAX1, SOX1 and ZNF582 in ESCC. The receiver operating characteristic curves showed that the detection of PAX1/SOX1/ZNF582 methylation status may serve as a promising biomarker for ESCC screening and diagnosis.
Highlights |
• | Gene methylation is one of the importance causes of cancer. |
• | The methylation levels of PAX1, SOX1, and ZNF582 genes were significantly higher in ESCC tissues than non-cancerous tissues. |
• | Hypermethylation of PAX1, SOX1 and ZNF582 was an independent risk factor of ESCC development. |
• | Detection of PAX1/SOX1/ZNF582 methylation status has excellent sensitivity and specificity of ESCC diagnosis. |
Abstract |
Background |
Esophageal squamous cell carcinoma (ESCC) is a highly invasive malignant tumor and the majority of patients have advanced stage of ESCC at diagnosis with poor outcome. Identification of sensitive and specific biomarkers for early screening of ESCC is critical for improving patient overall survival.
Methods |
Pyrosequencing was used to determine the magnitude of DNA methylation on the selected regions PAX1 (paired box gene1), SOX1(sex determining region Y-box-1), and ZNF582 (zinc finger protein 582) in ESCC.
Results |
The methylation levels ofPAX1, SOX1, and ZNF582 genes were significantly higher in the cancerous tissues compared to those in the non-cancerous (all P < 0.0001). The accuracy, sensitivity and specificity of PAX1 methylation for the detection of cancer were respectively 0.754, 96.0% and 51.4%; for SOX1 which were 0.781, 89.2% and 59.5%; for ZNF582 which were 0.898, 93.2% and 75.7%. Most importantly, both the sensitivity and specificity of ZNF582 methylation testing achieved 100% in female ESCC patients. Hypermethylation of PAX1/SOX1/ZNF582 exhibited as an independent risk factor for ESCC development. In addition, ZNF582 methylation level in tumor tissue from the female patients was higher than that from male patients, and it was higher in the moderate and poor differentiated tumors compared to that in well-differentiated tumors. SOX1 methylation level was significantly higher in tumors located in the upper region than those located in the middle or lower regions.
Conclusion |
The methylation levels ofPAX1, SOX1 and ZNF582 genes were all higher in cancer tissues than in paired-adjacent and normal tissues, suggesting that detection of PAX1/SOX1/ZNF582 methylation status may serve as a promising biomarker for ESCC screening and diagnosis.
Le texte complet de cet article est disponible en PDF.Abbreviations : ESCC, PAX1, SOX1, ZNF582, ASC, EC, SCC, AC, OS, C, U, SD, ROC, AUC, OR, CI
Keywords : Esophageal squamous cell carcinoma, PAX1, SOX1, ZNF582, DNA methylation
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Vol 120
Article 109488- décembre 2019 Retour au numéro
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