MicroRNA-1181 supports the growth of hepatocellular carcinoma by repressing AXIN1 - 01/10/19

Doi : 10.1016/j.biopha.2019.109397

Zewen Song ^a, Zhaomei Yu ^b, Limin Chen ^c, Zhijiao Zhou ^d, Qiong Zou ^d, Yang Liu ^d,^⁎
^a Department of Oncology, The Third Xiangya Hospital, Central South University, Changsha, 410013, China
^b Department of General Surgery, The Third Xiangya Hospital, Central South University, Changsha, 410013, China
^c Department of Infection, The Third Xiangya Hospital, Central South University, Changsha, 410013, China
^d Department of Pathology, The Third Xiangya Hospital, Central South University, Changsha, 410013, China

^⁎Corresponding author at: Department of Pathology, The Third Xiangya Hospital, Central South University, 138 Tongzipo Road, Yuelu District, Changsha, Hunan, 410013, China.Department of PathologyThe Third Xiangya HospitalCentral South University138 Tongzipo Road, Yuelu DistrictChangshaHunan410013China

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Abstract

Micro-RNAs regulate multiple biological behaviors of cancers, making them potential targets of new cancer therapies. MiR-1181 has been demonstrated to perform oncogenic or tumor-suppressing function in a tissue-dependent way, but its role in hepatocellular carcinoma (HCC) was unclear. Here, we showed that miR-1181 was significantly overexpressed in HCC tissues when compared with tumor-adjacent normal ones or normal liver tissues from donated organ, and that inhibition of miR-1181 could repress the growth of HCC cells. Through bioinformatics analysis and luciferase reporter assays, we found that axis inhibition protein 1 (AXIN1) was a direct target of miR-1181, and the expression of AXIN1 showed a negative correlation with that of miR-1181 in HCC. Therefore, these data indicated an oncogenic function of miRNA-1181 in the development of HCC and a potential target for the clinical treatment of HCC.

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Keywords : Hepatocellular carcinoma, miR-1181, AXIN1, Tumor growth

Plan

Introduction

Material and methods

Human tissue

In situ hybridization and semi-quantification

Cell culture and cell counting

Immunostainings

Vector constructs

Luciferase reporter assays

Western blotting analysis

Isolation of RNA and RT-qPCR

Results

miR-1181 was overexpressed in hepatocellular carcinoma

miR-1181 supported the growth of hepatocellular carcinoma

AXIN1 was putative target of miR-1181 and down-regulated in HCC

miR-1181 directly repressed the expression of AXIN1

Discussion

Author contributions

Funding

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Vol 119

Article 109397- novembre 2019 Retour au numéro

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MicroRNA-1181 supports the growth of hepatocellular carcinoma by repressing AXIN1 - 01/10/19

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