Metagenomic next-generation sequencing aids the diagnosis of viral infections in febrile returning travellers - 19/09/19
Highlights |
• | Next generation sequencing (NGS) has potential as an all-in-one diagnostic test for infection. |
• | Viral, bacterial and parasite genomes can be detected rapidly. |
• | NGS is becoming increasingly affordable but requires validation in clinical laboratories. |
Abstract |
Objectives |
Travel-associated infections are challenging to diagnose because of the broad spectrum of potential aetiologies. As a proof-of-principle study, we used MNGS to identify viral pathogens in clinical samples from returning travellers in a single center to explore its suitability as a diagnostic tool.
Methods |
Plasma samples from 40 returning travellers presenting with a fever of ≥38°C were sequenced using MNGS on the Illumina MiSeq platform and compared with standard-of-care diagnostic assays.
Results |
In total, 11/40 patients were diagnosed with a viral infection. Standard of care diagnostics revealed 5 viral infections using plasma samples; dengue virus 1 (n = 2), hepatitis E (n = 1), Ebola virus (n = 1) and hepatitis A (n = 1), all of which were detected by MNGS. Three additional patients with Chikungunya virus (n = 2) and mumps virus were diagnosed by MNGS only. Respiratory infections detected by nasal/throat swabs only were not detected by MNGS of plasma. One patient had infection with malaria and mumps virus during the same admission.
Conclusions |
MNGS analysis of plasma samples improves the sensitivity of diagnosis of viral infections and has potential as an all-in-one diagnostic test. It can be used to identify infections that have not been considered by the treating physician, co-infections and new or emerging pathogens.
Summary |
Next generation sequencing (NGS) has potential as an all-in-one diagnostic test. In this study we used NGS to diagnose returning travellers with acute febrile illness in the UK, highlighting cases where the diagnosis was missed using standard methods.
Le texte complet de cet article est disponible en PDF.Keywords : Returning travellers, NGS, Diagnosis
Plan
Vol 79 - N° 4
P. 383-388 - octobre 2019 Retour au numéro
Article précédent
- Comparative global epidemiology of influenza, respiratory syncytial and parainfluenza viruses, 2010–2015
- Tommy T Lam, Julian W Tang, Florence Y Lai, Hassan Zaraket, Ghassan Dbaibo, Seweryn Bialasiewicz, Sarah Tozer, Jean-Michel Heraud, Steven J Drews, Todd Hachette, Paul KS Chan, Evelyn SC Koay, Hong Kai Lee, Kok Keng Tee, Yang Liu, Pieter LA Fraaij, Lance Jennings, Matti Waris, Mel Krajden, André Corriveau, Hamid Jalal, Hidekazu Nishimura, Pagbajabyn Nymadawa, Darmaa Badarch, Aripuana Watanabe, Alice Kabanda, Theo Sloots, Jen Kok, Dominic E Dwyer, Marion Koopmans, INSPIRE (International Network for the Sequencing of Respiratory Viruses)
- Elevated lactate levels in the cerebrospinal fluid associated with bacterial meningitis
- Andrew Bosworth, Khiranmai Bhatt, Alexandra Nance, Gemma Winzor, Sowsan F. Atabani
Bienvenue sur EM-consulte, la référence des professionnels de santé.
L’accès au texte intégral de cet article nécessite un abonnement.
Déjà abonné à cette revue ?