Expression of GR-α and HDAC2 in steroid-Sensitive and steroid-Insensitive interstitial lung disease - 18/09/19
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Highlights |
• | NF-κB is over-expressed in lung tissue cells of IPF patients reveal a serious inflammatory reaction. |
• | GR-α is nearly absent in lung tissue cells of IPF patients, which may explain why IPF does not respond to glucocorticoid therapy. |
• | High expression of HDAC2 in lung tissue cells of COP patients, which may predicts glucocorticoid sensitivity. |
Abstract |
Background |
Corticosteroid is one of the main treatments for interstitial lung disease (ILD). Cryptogenic-organizing pneumonia (COP) is sensitive to corticosteroid therapy, whereas idiopathic pulmonary fibrosis (IPF) is not. Glucocorticoid receptor-α (GR-α) and histone deacetylase 2 (HDAC2) play critical roles in the sensitivity to corticosteroid therapy; however, it is unclear whether HDAC2 and/or GR-α are expressed in the lung tissues of patients with COP and/or IPF. Possible aberrant expressions of HDAC2 and GR-α in IPF and COP were investigated in the current study.
Methods |
Lung tissue samples were obtained from patients with COP (n = 9), IPF (n = 8), pulmonary abscesses (n = 7), or pulmonary inflammatory pseudotumors (n = 6) before corticosteroid treatment, as well as from control subjects (n = 10). The expression of GR-α, HDAC2, PI3K-δ, and NF-κBp65 in the samples was assessed by immunohistochemistry.
Results |
GR-α expression was the same in lung tissues from COP patients and control subjects, but was significantly lower in lung tissue from IPF. In addition, HDAC2 was significantly higher in lung tissues of COP patients compared to both IPF and control subjects. Furthermore, the transcription factor NF-κBp65 was significantly lower in lung tissues from both COP and control compared to IPF subjects, whereas there was no difference in NF-κBp65 when comparing tissues from COP patients to controls. HDAC2 and GR-α were negatively correlated with NF-κBp65 in COP lung tissue.
Conclusion |
HDAC2 and GR-α expression in lung tissues are potential biomarkers for predicting corticosteroid sensitivity when initially treating COP and IPF, as well as other forms of ILD.
Le texte complet de cet article est disponible en PDF.Abbreviations : ILD, GR-α, HDAC2, COP, IPF, GRE, PA, PIP, PI3K-δ, FEV1, FVC
Keywords : Interstitial lung disease, Glucocorticoid receptor-α, Histone deacetylases-2, Cryptogenic-organizing pneumonia, Idiopathic pulmonary fibrosis
Plan
Vol 118
Article 109380- octobre 2019 Retour au numéro
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