LncRNA MNX1-AS1 promotes progression of esophageal squamous cell carcinoma by regulating miR-34a/SIRT1 axis - 16/06/19
, Hongle Li b, 1 , Yurong Xing c , Jinlin Jia a , Jinxiu Sheng a , Lijun Yang a , Kaiyan Sun a , Yunhui Qu a , Yan Zhang a , Huiqing Yin a , Junhu Wan a, ⁎ , Fucheng He a, ⁎ Bienvenue sur EM-consulte, la référence des professionnels de santé.
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Highlights |
• | MNX1-AS1 that was associated with ESCC lymph node metastasis, is significantly upregulated in ESCC tissues. |
• | MNX1-AS1 can promote the ESCC cells proliferation, migration, and invasion capacity, and it can regulete ESCC cell cycle and apoptosis progression. |
• | MNX1-AS1 promotes progression of esophageal squamous cell carcinoma by regulating miR-34a/SIRT1 axis. |
Abstract |
Background |
Long non-coding RNAs (lncRNAs) are powerful factors influencing the tumorigenesis and metastasis of multiple carcinomas. LncRNA MNX1-AS1 plays critical roles in the progression of tumor formation according to recent research, while its roles in esophageal squamous cell carcinoma (ESCC) remains unknown.
Methods |
The expression levels of lncRNA MNX1-AS1 were examined in ESCC tissues by quantitative reverse transcriptase polymerase chain reaction (qRT-PCR). The role of lncRNA MNX1-AS1 was performed by WST-1 proliferation assays, migration and invasion assays. Besides, the molecular mechanism of lncRNA MNX1-AS1 was verified by online bioinformatics, qRT-PCR and rescue assays.
Results |
MNX1-AS1 was signifcantly upregulated in ESCC tissues. It was conformed that high MNX1-AS1 expression was associated with ESCC lymph node metastasis. Moreover, we found that knockdown of MNX1-AS1 apparently suppressed the cell proliferation, migration, and invasion capacity. Flow cytometry analysis showed MNX1-AS1 regulated ESCC cell cycle and apoptosis progression. Mechanism analysis revealed that miR-34a inhibitor could rescue the influence of inhibiting MNX1-AS1 on ESCC cells migration by serving as competing endogenous RNA (ceRNAs). Furthermore, we found that miR-34a specifically targeted SIRTI.
Conclusions |
Taken together, we demonstrated that lncRNA MNX1-AS1/miR-34a/SIRT1 regulatory axis could play an important role in ESCC progression, and MNX1-AS1 may act as a novel potential biomarker for esophageal squamous cell carcinoma.
Le texte complet de cet article est disponible en PDF.Keywords : Esophageal squamous cell carcinoma, Long non-coding RNA, MNX1-AS1, MiR-34a, SIRT1
Plan
Vol 116
Article 109029- août 2019 Retour au numéro
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