Plumbagin ameliorates liver fibrosis via a ROS-mediated NF-кB signaling pathway in vitro and in vivo - 16/06/19
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Graphical abstract |
The mechanism of Plumbagin ameliorates liver fibrosis via a ROS-mediated NF-кB signaling pathway. The oxidative stress and the NF-κB signaling pathway are involved in the process of CCl4-induced rat liver fibrosis and that IL-1β-induces HSCs and PL effectively suppresses liver fibrosis via a ROS-mediated NF-кB signaling pathway.
Highlights |
• | Plumbagin significantly prevents CCl4-induced liver fibrosis, as evidenced by attenuated histopathological changes. |
• | In vitro and in vivo regulation of oxidative stress and expression level of NF-κB inflammatory pathway by plumbagin. |
• | Plumbagin significantly reduced ROS expression in IL-1β-stimulated HSCs and inhibited NF-κB p65 nuclear translocation. |
Abstract |
Aims |
The purpose of this study was to investigate plumbagin (PL) on liver fibrosis in vitro and in vivo and to explore the underlying mechanisms.
Methods |
Carbon tetrachloride (CCl4) was used to establish a rat liver fibrosis model, primary hepatic stellate cells (HSCs) were isolated from the rat liver, and fibrosis-related indicators were detected.
Results |
The results revealed that PL significantly prevented CCl4-induced liver fibrosis, as evidenced by the attenuation of histopathological changes, the decrease of MDA and the increase of SOD and GSH-P X . In addition, PL downregulated the mRNA levels of NOX4 and procollagen I; the protein expression levels of NOX4 and p-IκB; and the transcriptional activity of NF-κB in liver fibrosis rats. Moreover, PL significantly decreased ROS expression, protein expression of α-SMA and collagen III, and activation of NF-κB and inhibited the nuclear translocation of NF-κB p65 in IL-1β-stimulated HSCs in vitro.
Conclusion |
The results of our study indicate that PL can mitigate liver fibrosis in vitro and in vivo, which may be related to the ROS-mediated NF-кB signaling pathway.
Le texte complet de cet article est disponible en PDF.Keywords : Plumbagin, Liver fibrosis, ROS, NF-кB signaling pathway
Plan
Vol 116
Article 108923- août 2019 Retour au numéro
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