Imatinib-induced changes in the expression profile of microRNA in the plasma and heart of mice—A comparison with doxorubicin - 09/06/19
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Highlights |
• | Several circulating microRNAs were affected by imatinib treatment in mice. |
• | Most circulating miRNAs affected by DOX were affected by IMB with the same trend. |
• | Mir-34a was the only miRNA upregulated by DOX in mice hearts. |
• | MiR-205 was downregulated in mice hearts treated by IMB as well as DOX. |
Abstract |
Cardiotoxicity is a serious adverse reaction to cancer chemotherapy and may lead to critical heart damage. Imatinib mesylate (IMB), a selective tyrosine kinase inhibitor, is sometimes accompanied by severe cardiovascular complications. To minimize risk, early biomarkers of such complications are of utmost importance. At the present time, microRNAs (miRNAs) are intensively studied as potential biomarkers of many pathological processes. Many miRNAs appear to be specific in some tissues, including the heart. In the present study we have explored the potential of specific miRNAs to be early markers of IMB-induced cardiotoxicity. Doxorubicin (DOX), an anthracycline with well-known cardiotoxicity, was used for comparison. NMRI mice were treated with IMB or DOX for nine days in doses corresponding to the highest recommended doses in oncological patients, following which plasmatic levels of miRNAs were analyzed in miRNA microarrays and selected cardio-specific miRNAs were quantified using qPCR. The plasmatic level of miR-1a, miR-133a, miR-133b, miR-339, miR-7058, miR-6236 and miR-6240 were the most different between the IMB-treated and control mice. Interestingly, most of the miRNAs affected by DOX were also affected by IMB showing the same trends. Concerning selected microRNAs in the hearts of individual mice, only miR-34a was significantly increased after DOX treatment, and only miR-205 was significantly decreased after IMB and DOX treatment. However, no changes in any miRNA expression correlated with the level of troponin T, a classical marker of heart injury.
Le texte complet de cet article est disponible en PDF.Abbreviations : DOX, IMB, miRNA, TnT
Keywords : MicroRNA, Imatinib, Doxorubicin, Biomarker, Cardiotoxicity
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Vol 115
Article 108883- juillet 2019 Retour au numéroBienvenue sur EM-consulte, la référence des professionnels de santé.
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