Toxoplasma gondii causes increased ICAM-1 and serotonin expression in the jejunum of rats 12 h after infection - 17/05/19
, Letícia Sarturi Pereira-Severi a, Carlos Alberto Miqueloto c, João Luís Garcia d, Gessilda de Alcântara Nogueira-Melo a, Débora de Mello Gonçales Sant’Ana aBienvenue sur EM-consulte, la référence des professionnels de santé.
L’accès au texte intégral de cet article nécessite un abonnement.
| pages | 8 |
| Iconographies | 7 |
| Vidéos | 0 |
| Autres | 0 |
Graphical abstract |
Highlights |
• | 5-HT is a neuroendocrine mediator present in the intestinal mucosa of all vertebrates. |
• | T. gondii enhances 5-HT expression in enterochromaffin cells. |
• | T. gondii increases the expression of ICAM-1 in the intestinal mucosa. |
• | T. gondii causes changes in the jejunal mucosa in less than 24 h of infection. |
• | Collagen remodeling and mastocytosis are observed in the jejunum of infected rats. |
Abstract |
Objective: To analyze the remodeling dynamics of total collagen, type I and III, the expression of ICAM-1 and 5-HT in the jejunum of rats.
Methods: Twenty-eight Wistar rats were randomly assigned to two experimental groups: the control group (CG, n = 7) and the infected group (receiving 5,000 sporulated T. gondii oocysts - ME49 strain, genotype II, n = 21). Seven infected rats each at 6 (G6), 12 (G12), and 24 (G24) hours post infection were sacrificed and segments of jejunum were collected for standard histological, histochemical, and immunohistochemistry processing techniques.
Results: The infection promoted ICAM-1 and 5-HT expression, type III collagen, and total mast cell increases. However, it also caused a reduction in the area occupied by type I collagen fibers, and in submucosa thickness, and caused ganglion and peri-ganglion alterations.
Conclusion: The structural damage caused by toxoplasmic infection is intense during the first 24 h post inoculation. At peak dissemination, from 12 to 24 h, there is an increase in ICAM-1 and 5-HT expression, with intense migration of mast cells to the site of infection. There was also a reduction in submucosa thickness, and an effective loss of extracellular matrix (ECM) organization, which included changes in the dynamics of type I and III total collagen deposition.
Le texte complet de cet article est disponible en PDF.Keywords : Mast cells, Enterochromaffin cells, Adhesion molecules, Toxoplasmosis
Plan
Vol 114
Article 108797- juin 2019 Retour au numéro
Article précédent
- Protective effect of glycine in streptozotocin-induced diabetic cataract through aldose reductase inhibitory activity
- Wei Li, Yujie Zhang, Na Shao
- Nasopharyngeal cancer combination chemoradiation therapy based on folic acid modified, gefitinib and yttrium 90 co-loaded, core-shell structured lipid-polymer hybrid nanoparticles
- Fu Yugui, Hailan Wang, Dezhong Sun, Xiaoyan Zhang
Bienvenue sur EM-consulte, la référence des professionnels de santé.
L’accès au texte intégral de cet article nécessite un abonnement.
Bienvenue sur EM-consulte, la référence des professionnels de santé.
L’achat d’article à l’unité est indisponible à l’heure actuelle.
Déjà abonné à cette revue ?