Oxymatrine ameliorates agomelatine-induced hepatocyte injury through promoting proteasome-mediated CHOP degradation - 17/05/19


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Graphical abstract |
Abstract |
Background |
The novel antidepressant drug agomelatine has been observed to cause adverse effect of hepatotoxicity in clinical applications. This study was designed to explore protective agents and investigated the underlying mechanism on L02 cells.
Method |
L02 cells were treated with agomelatine and oxymatrine (OMT) and cell apoptosis were analyzed through flow cytometric analysis, CCK-8 assay and TUNEL assay. In a separate experiment, the expressions of ER stress-related proteins were determined by western blot. In addition, MG132, chloroquine (CQ) and bafilomycinA1(BafA1) were used to investigate the potential pathway participating in CHOP degradation.
Results |
OMT significantly rescued agomelatine-induced hepatocyte apoptosis. Agomelatine treatment resulted in accumulation of CHOP protein in L02 cells, and this phenomenon could be significantly reduced by OMT, whereas abolished by MG132 treatment.
Conclusion |
We have demonstrated for the first time that OMT ameliorates the hepatocyte toxicity induced by agomelatine through decreasing CHOP on protein level. The underlying mechanism was proved to involve the molecular events that OMT promotes CHOP degradation via proteasome pathway. Overall, these results suggest that using OMT in combination with agomelatine may provide a safety strategy for clinical depression treatment.
Le texte complet de cet article est disponible en PDF.Abbreviations : ALT, AST, ATF6, BafA1, CCK-8, CETSA, CQ, ER, IRE1α, ISRIB, MAO, OMT, PERK, SEM, SNRIs, SSRIs, TUNEL, DMEM, SDS-PAGE, DAPI, one-way ANOVA
Keywords : Oxymatrine, CHOP degradation, Hepatocyte injury, ER stress, Agomelatine
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Vol 114
Article 108784- juin 2019 Retour au numéroBienvenue sur EM-consulte, la référence des professionnels de santé.
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