Platelet-rich plasma (PRP) is a novel therapeutic treatment option for joint and tendon disease, but preparation methods are varied. This article summarizes research on uses of PRP, compares classification systems to standardize various PRP compositions, and discusses the most common methodologies to produce PRP. Even with advances in understanding PRP, there are unknowns about the factors and processes that may have an impact on treatment efficacy for musculoskeletal conditions. PRP studies should pursue determining optimal PRP preparation, setting a standard to evaluate PRP mixtures and preparation methods, assessing efficacy of PRP for various musculoskeletal conditions, and managing and reducing costs.