GM-CSF intrinsically controls eosinophil accumulation in the setting of allergic airway inflammation - 04/04/19
Abstract |
Background |
Eosinophils are a therapeutic target in asthmatic patients, and GM-CSF has been suggested to control various aspects of eosinophil biology, including development, function, and survival. However, to date, the role of GM-CSF signaling in eosinophils in vivo is largely unclear.
Objective |
We sought to elucidate the role of GM-CSF signaling in asthmatic inflammation.
Methods |
Wild-type and GM-CSF receptor α (Csf2ra)–deficient mice reconstituted with Csf2ra-proficient alveolar macrophages were subjected to different models of airway inflammation to evaluate the effect of GM-CSF signaling deficiency on asthmatic inflammation in general and on eosinophils in particular.
Results |
We demonstrate that GM-CSF signaling, although being largely dispensable for eosinophil development at steady state, intrinsically promotes accumulation of eosinophils in the lung during allergic airway inflammation. In contrast, chitin-induced eosinophil accumulation in the peritoneal cavity occurs independent of GM-CSF, indicating organ specificity. We show that GM-CSF induces chemokinesis and promotes eosinophil survival in vitro, which likely contribute to eosinophil accumulation in the airways in vivo.
Conclusion |
GM-CSF intrinsically promotes eosinophil accumulation in the setting of pulmonary allergic inflammation.
Le texte complet de cet article est disponible en PDF.Graphical abstract |
Key words : Eosinophils, asthma, allergic airway inflammation, GM-CSF, chemokinesis
Abbreviations used : AM, BAL, BM, DC, EdU, FACS, GM-CSFR, HDM, OVA, PAP, WT
Plan
| Supported by Swiss National Science Foundation (SNCF) Grant 310030_163443. |
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| Disclosure of potential conflict of interest: The authors declare that they have no relevant conflicts of interest. |
Vol 143 - N° 4
P. 1513 - avril 2019 Retour au numéro
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