Serial testing of Mycobacterium tuberculosis infection in Chinese village doctors by QuantiFERON-TB Gold Plus, QuantiFERON-TB Gold in-Tube and T-SPOT.TB - 24/03/19

Doi : 10.1016/j.jinf.2019.01.008

Haoran Zhang ^a,^{^{1
These authors contributed equally to this work.}}, Henan Xin ^a,^{^{1
These authors contributed equally to this work.}}, Dakuan Wang ^b,^{^{1
These authors contributed equally to this work.}}, Shouguo Pan ^b, Zisen Liu ^b, Xuefang Cao ^a, Jinxing Wang ^c, Xiangwei Li ^a, Boxuan Feng ^a, Mufei Li ^a, Qianting Yang ^d, Mingxia Zhang ^d, Qi Jin ^a, Lei Gao ^a,^⁎
^a NHC Key Laboratory of Systems Biology of Pathogens, Institute of Pathogen Biology, and Center for Tuberculosis Research, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, P.R. China
^b Zhongmu County Center for Diseases Control and Prevention, Zhongmu 451450, China
^c Zhongmu County Health Commission, Zhongmu 451450, China
^d Guangdong Key Laboratory for Diagnosis & Treatment of Emerging Infectious Diseases, Shenzhen Third People's Hospital, Shenzhen University School of Medicine, Shenzhen 518112, China

^⁎Corresponding Author

Summary

Objectives

To evaluate the performance of QuantiFERON-TB Gold Plus (QFT-Plus) on Mycobacterium tuberculosis (MTB) infection test among registered village doctors from China.

Methods

MTB infection of the registered village doctors in Zhongmu County were tested using QFT-Plus and two other interferon-gamma release assays (IGRAs) in parallel: QuantiFERON-TB Gold In-Tube (QFT) and T-SPOT.TB (T-SPOT). Retests were carried out for baseline positives at 3 and 6 months later, respectively.

Results

A total of 616 village doctors were included in the baseline examination. The positivity of QFT, QFT-Plus and T-SPOT was 27.91% (168/602), 31.22% (187/599) and 27.70% (169/610), respectively. The concordance between QFT and QFT-Plus was 94.81% (Kappa coefficient: 0.87) and between T-SPOT and QFT-Plus was 88.93% (Kappa coefficient: 0.73). Reversions were frequently observed for all three assays. With respect to QFT-Plus, the quantitative results of reversions in the serial testing were mostly distributed in an “uncertain range” zone (0.2-0.7 IU/mL). Similar patterns of distribution were observed for QFT and T-SPOT as well.

Conclusion

Village doctors should gain more attention as an at-risk group for TB infection control in rural China. Our results support, by means of serial testing, a good agreement between QFT-Plus and QFT in Chinese population.

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Keywords : MTB infection, Village doctors, Serial testing, IGRAs, QFT-Plus

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Vol 78 - N° 4

P. 305-310 - avril 2019 Retour au numéro

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Serial testing of Mycobacterium tuberculosis infection in Chinese village doctors by QuantiFERON-TB Gold Plus, QuantiFERON-TB Gold in-Tube and T-SPOT.TB - 24/03/19

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