MicroRNA hsa-miR-29a-3p is a plasma biomarker for the differential diagnosis and monitoring of tuberculosis - 31/01/19

Doi : 10.1016/j.tube.2018.12.001

Elvis Ndukong Ndzi ^a,^b,^c , Céline Nguefeu Nkenfou ^b,^d,^⁎ , Linda Mouafo Mekue ^a,^b,^c , Lorena Zentilin ^e , Ousman Tamgue ^f,^g,^h , Eric Walter Yone Pefura ^i,^j , Jules-Roger Kuiaté ^a , Mauro Giacca ^e , Alexis Ndjolo ^b,^j
^a Department of Biochemistry, University of Dschang, P.O. BOX 56, Dschang, Cameroon
^b Chantal Biya International Reference Centre (CBIRC), P.O BOX 3077, Yaounde, Cameroon
^c Laboratory of Animal Physiology and Health, Institute of Agricultural Research for Development (IRAD), P.O BOX 51, Bambui, Cameroon
^d Higher Teachers' Training College, University of Yaounde I, P.O. BOX 47, Yaounde, Cameroon
^e International Centre for Genetic Engineering and Biotechnology (ICGEB), Padriciano, 99, 34149, Trieste, Italy
^f University of Cape Town, Institute of Infectious Diseases and Molecular Medicine (IDM), Division of Immunology and South Africa Medical Research Council, Immunology of Infectious Disease, Faculty of Health Sciences, Cape Town, 7925, South Africa
^g International Centre for Genetic Engineering and Biotechnology (ICGEB), Cape Town Component, Cape Town, 7925, South Africa
^h Department of Biochemistry, Faculty of Sciences, University of Douala, P.O BOX 24157, Douala, Cameroon
ⁱ Jamot Hospital, Yaounde, Cameroon
^j Faculty of Medicine and Biomedical Science, University of Yaounde I, P.O BOX 1364, Yaounde, Cameroon

^∗Corresponding author. Head Systems Biology Laboratory CBIRC, P.O BOX 3077, Yaounde, Cameroon.Head Systems Biology Laboratory CBIRCP.O BOX 3077YaoundeCameroon

Abstract

The diagnosis of tuberculosis (TB) continues to pose substantial public health problems. The quest for diagnostic biomarkers for TB is therefore primordial. This study aimed to evaluate the diagnostic and anti-TB treatment monitoring potentials of some selected miRNAs. Quantitative real time polymerase chain reaction and Receiver operating characteristics were used to estimate the ability of miRNAs to discriminate between healthy controls (HEC), latent (LTB) and active TB (ATB). The study showed that: hsa-miR-29a-3p, hsa-miR-155-5p and hsa-miR-361-5p were significantly upregulated in ATB compared to HEC while hsa-miR-29a-3p, and hsa-miR-361-5p were also significantly up-regulated in ATB compared to LTB (all P ≤ 0.05). MiR-29a-3p showed a good (81.37%) distinguishing performance in discriminating ATB from HEC and a good (84.35%) diagnostic performance in discriminating ATB from LTB. The performance of miR-29a-3p present in the blood in discriminating active TB from latent TB and healthy controls indicates it may be a useful biomarker for diagnosis of TB. Because this miRNA is found in blood (plasma) which is easy to collect compared to sputum it could be used in pediatric and extra-pulmonary TB cases.

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Keywords : microRNA, Biomarker, Pediatric/extra-pulmonary tuberculosis, Diagnosis, Performance

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Vol 114

P. 69-76 - janvier 2019 Retour au numéro

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MicroRNA hsa-miR-29a-3p is a plasma biomarker for the differential diagnosis and monitoring of tuberculosis - 31/01/19

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