Accuracy of the color plate micro-colony detection for the diagnosis of Mycobacterium tuberculosis complex in Northwest Ethiopia - 31/01/19

Doi : 10.1016/j.tube.2018.11.007

Agumas Shibabaw ^a,^⁎ , Baye Gelaw ^a, Holden Kelley ^b, Joan Miquel Balada-Llasat ^c, Carlton Evans ^d, Shu-Hua Wang ^e, Jordi B. Torrelles ^f, Belay Tessema ^a
^a Department of Medical Microbiology, School of Biomedical and Laboratory Sciences, College of Medicine and Health Sciences, University of Gondar, Gondar, Ethiopia
^b Department of Microbial Infection and Immunity, The Ohio State University, Columbus, OH, USA
^c Department of Pathology, College of Medicine, The Ohio State University, Columbus, OH, USA
^d The Wellcome Centre for Clinical Tropical Medicine, Imperial College of London, London, United Kingdom
^e Department of Internal Medicine, Division of Infectious Diseases, College of Medicine, The Ohio State University, Columbus, OH, USA
^f Texas Biomedical Research Institute, San Antonio, TX, USA

^∗Corresponding author. P.O.Box: 196, EthiopiaP.O.Box: 196Ethiopia

Abstract

Background

Accurate and timely tuberculosis diagnosis is the primary step for initiating effective treatment. The color plate agar-based culture test (TB-CX test) is low cost, simple to use and detects Mycobacterium tuberculosis faster. Therefore, the main objective of this study was to compare the diagnostic accuracy and time to detection of positive cultures using color test and Lӧwenstein Jensen culture.

Methods

A comparative cross-sectional study was conducted at University of Gondar Hospital. A total of 200 sputum samples were collected from TB patients and processed for direct smear microscopy and cultures.

Results

Sixty-five percent were found positive on both methods and 4 (2%) were positive on LJ culture and negative on the color plate. The median time for detection of MTB growth was significantly shorter using color plate test (Median 12 days) than LJ culture (Median 21 days) (P < 0.0001). The overall sensitivity and specificity of the color test compared to LJ culture were 97% (95% CI: 93–99) and 100% (95% CI: 94–100), respectively.

Conclusions

The color plate test for micro-colonies allows early and accurate MTB diagnosis in a median time of 12 days. This rapid method could be an option for diagnosis of pulmonary TB in resource limited settings.

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Keywords : Tuberculosis, Diagnostics, Color plate, TB-CX test, Micro-colony, Löwenstein-Jensen

Plan

Introduction

Materials and methods

Study design and setting

Data collection, transportation and quality control

Culture and identification of M. tuberculosis complex

Sample size determination

Statistical analysis

Ethical approval

Results

Demographic and clinical characteristics

Accuracy and time to micro-colony detection of color TB-CX test

Discussion

Conflict of interest

Funding

Authors' contribution

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Vol 114

P. 54-60 - janvier 2019 Retour au numéro

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Accuracy of the color plate micro-colony detection for the diagnosis of Mycobacterium tuberculosis complex in Northwest Ethiopia - 31/01/19

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