Down-regulation of miR-500 and miR-628 suppress non-small cell lung cancer proliferation, migration and invasion by targeting ING1 - 13/11/18
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| pages | 12 |
| Iconographies | 10 |
| Vidéos | 0 |
| Autres | 0 |
Graphical abstract |
Highlights |
• | miR-500 and miR-628 suppress NSCLC proliferation and metastasis. |
• | miR-500 and miR-628 functioned as a direct target for ING1. |
• | ING1 could dramatically inhibit NSCLC cells proliferation and metastasis. |
Abstract |
Background |
MicroRNAs (miRNAs) have been consistently demonstrated to be involved in non-small cell lung cancer (NSCLC) as either tumor oncogenes or tumor suppressors. However, the detailed role of miR-500 and miR-628 in NSCLC remain poorly understood.
Methods |
The expressions of miR-500 and miR-628 in NSCLC tissues and cell lines were measured by quantitative real-time PCR (qRT-PCR). Cells migration, invasion, proliferation, adhesion and apoptosis abilities were test to analyze the biological functions of miR-500 and miR-628 in NSCLC. A bioinformatic analysis was conducted to predict the target genes regulated by miR-500 and miR-628 using TargetScan (mamm/). Luciferase reporter assay was employed to validate the direct targeting of ING1 by miR-500 and miR-628.
Results |
In this study, miR-500 and miR-628 were up-regulated with NSCLC tissues. Furthermore, inhibition of miR-500 and miR-628 significantly suppressed NSCLC cells proliferation, migration, invasion and adhesion, and induced NSCLC cells apoptosis. Additionally, the result showed that ING1 functioned as the direct target for miR-500 and miR-628, which was a core tumor suppressor in regulating NSCLC progression. Over-expression of ING1 could dramatically inhibit NSCLC cells proliferation, migration and invasion, and promote cells apoptosis.
Conclusion |
These results brought new insights into the oncogenic role of miR-500 and miR-628 in NSCLC, indicating that miR-500 and miR-628 might be the novel biomarkers for the diagnosis and prognosis of NSCLC.
Le texte complet de cet article est disponible en PDF.Keywords : miR-500, miR-628, ING1, NSCLC, Proliferation, Migration
Plan
Vol 108
P. 1628-1639 - décembre 2018 Retour au numéro
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