The nasal microbiome in asthma - 06/09/18
Abstract |
Background |
Nasal microbiota may influence asthma pathobiology.
Objective |
We sought to characterize the nasal microbiome of subjects with exacerbated asthma, nonexacerbated asthma, and healthy controls to identify nasal microbiota associated with asthma activity.
Methods |
We performed 16S ribosomal RNA sequencing on nasal swabs obtained from 72 primarily adult subjects with exacerbated asthma (n = 20), nonexacerbated asthma (n = 31), and healthy controls (n = 21). Analyses were performed using Quantitative Insights into Microbial (QIIME); linear discriminant analysis effect size (LEfSe); Phylogenetic Investigation of Communities by Reconstruction of Unobserved States; and Statistical Analysis of Metagenomic Profiles (PICRUSt); and Statistical Analysis of Metagenomic Profiles (STAMP). Species found to be associated with asthma activity were validated using quantitative PCR. Metabolic pathways associated with differentially abundant nasal taxa were inferred through metagenomic functional prediction.
Results |
Nasal bacterial composition significantly differed among subjects with exacerbated asthma, nonexacerbated asthma, and healthy controls (permutational multivariate ANOVA, P = 2.2 × 10−2). Relative to controls, the nasal microbiota of subjects with asthma were enriched with taxa from Bacteroidetes (Wilcoxon-Mann-Whitney, r = 0.33, P = 5.1 × 10−3) and Proteobacteria (r = 0.29, P = 1.4 × 10−2). Four species were differentially abundant based on asthma status after correction for multiple comparisons: Prevotella buccalis, Padj = 1.0 × 10−2; Dialister invisus, Padj = 9.1 × 10−3; Gardnerella vaginalis, Padj = 2.8 × 10−3; Alkanindiges hongkongensis, Padj = 2.6 × 10−3. These phyla and species were also differentially abundant based on asthma activity (exacerbated asthma vs nonexacerbated asthma vs controls). Quantitative PCR confirmed species overrepresentation in asthma relative to controls for Prevotella buccalis (fold change = 130, P = 2.1 × 10−4) and Gardnerella vaginalis (fold change = 160, P = 6.8 × 10−4). Metagenomic inference revealed differential glycerolipid metabolism (Kruskal-Wallis, P = 1.9 × 10−4) based on asthma activity.
Conclusions |
Nasal microbiome composition differs in subjects with exacerbated asthma, nonexacerbated asthma, and healthy controls. The identified nasal taxa could be further investigated for potential mechanistic roles in asthma and as possible biomarkers of asthma activity.
Le texte complet de cet article est disponible en PDF.Graphical abstract |
Key words : Nasal, microbiome, 16S rRNA sequencing, asthma, upper respiratory tract, upper airway
Abbreviations used : LEfSe, OTU, PERMANOVA, qPCR, rRNA
Plan
| This study was supported by the National Institutes of Health (K08AI093538, R01AI118833). Computational infrastructure provided by the Department of Scientific Computing at the Icahn School of Medicine at Mount Sinai. |
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| Disclosure of potential conflict of interest: J. Andrade and S. Bunyavanich report receiving grants from the National Institutes of Health/National Institute of Allergy and Infectious Diseases. The rest of the authors declare that they have no relevant conflicts of interest. |
Vol 142 - N° 3
P. 834 - septembre 2018 Retour au numéro
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