Downregulation of miR-218-5p promotes invasion of oral squamous cell carcinoma cells via activation of CD44-ROCK signaling - 25/08/18

Doi : 10.1016/j.biopha.2018.06.151

Xiaojie Li ^a, Jianya He ^a, Miaomiao Shao ^a, Bai Cui ^b, Fei Peng ^b, Jiao Li ^a, Yan Ran ^a, Dong Jin ^a, Jing Kong ^a, Jinming Chang ^b, Liqiang Duan ^b, Xuesong Yang ^c, Yong Luo ^d, Yao Lu ^e, Bingcheng Lin ^e, Tingjiao Liu ^a,^⁎
^a Department of Oral Pathology, College of Stomatology, Dalian Medical University, Dalian, China
^b Institute of Cancer Stem Cell, Dalian Medical University, Dalian, China
^c Department of Biochemistry and Molecular Biology, Liaoning Provincial Core Lab of Glycobiology and Glycoengineering, Dalian Medical University, Dalian, China
^d Faculty of Chemical, Environmental and Biological Science and Technology, Dalian Technology University, Dalian, China
^e Department of Biotechnology, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, China

^⁎Corresponding author at: Department of Oral Pathology, College of Stomatology, Dalian Medical University, Dalian 116044, China.Department of Oral Pathology, College of Stomatology, Dalian Medical University, Dalian 116044China

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Highlights

•	Isolate the invasion front cells of the OSCC using microfluidic chip.
•	Invasion front cells show high invasive capacity and decrease of miR-218-5p.
•	Downregulation of miR-218-5p promotes OSCC invasion by targeting CD44–ROCK pathway.

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Abstract

The invasion front of oral squamous cell carcinoma (OSCC) harbors the most aggressive cells of the tumor and is critical for cancer invasion and metastasis. MicroRNAs (miRNAs) play important roles in OSCC progression. In this study, we modelled the OSCC invasion front on a microfluidic chip, and investigated differences in miRNA profiles between cells in the invasion front and those in the tumor mass by small RNA sequencing. We found that miR-218-5p was downregulated in invasion front cells and negatively regulates OSCC invasiveness by targeting the CD44–ROCK pathway. Thus, miR-218-5p may serve as a useful therapeutic target for OSCC. Moreover, invasion front cell isolation based-on microfluidic technology provided a useful strategy for cancer invasion study.

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Keywords : miR-218-5p, OSCC, Invasion, CD44, ROCK, Microfluidic chip

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Vol 106

P. 646-654 - octobre 2018 Retour au numéro

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Downregulation of miR-218-5p promotes invasion of oral squamous cell carcinoma cells via activation of CD44-ROCK signaling - 25/08/18

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