Puerarin promoted proliferation and differentiation of dopamine-producing cells in Parkinson’s animal models - 25/08/18
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Graphical abstract |
Highlights |
• | In vitro, PR promotes the differentiation of TH+ cells from MSCs. |
• | In vivo, PR promotes the survival of transplantated DA-producing cells. |
• | In vivo, PR promoted the transplanted cells proliferated and differentiated into TH + cells. |
Abstract |
Background |
Parkinson’s disease (PD) is caused by the gradual loss of dopamine-producing cells in the brain. This study evaluated the potential neuroprotective role of puerarin (PR) on dopamine (DA)-producing cells in vitro and in vivo.
Method |
In vitro, the effects of PR on proliferation and differentiation and DA releases of mesenchymal stem cells (MSCs) were assayed by CCK-8, flow cytometry, real-time PCR and ELISA respectively. Then the differentiated cells were labeled with enhanced green fluorescent protein (EGFP) and administrated into PD animal models induced by 6-OHDA. The proliferation and differentiation of labeled cells were identified by fluorescence microscopy and immunostaining.
Results |
In vitro, after being treated with different concentrations of PR for 1 week, the TUJ1, TH and DAT protein and mRNA expression and DA releases increased significantly. In vivo, after transplantation of PR-treated DA-producing cells, the symptoms of PD improved significantly from the second week after transplantation; more transplanted cells survived and migrated to wider region along injection line; more transplanted cells proliferated and differentiated into TH+ cells; more DA was detected in the striatum during 6 weeks’ observation.
Conclusion |
The results suggest that PR promote DA neuron survival, proliferation and differentiation.
Le texte complet de cet article est disponible en PDF.Keywords : Parkinson’s disease, Mesenchymal stem cells, Puerarin, Stem cell transplantation, DA neuron
Plan
Vol 106
P. 1236-1242 - octobre 2018 Retour au numéro
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