IP-10 and RANTES as biomarkers for pulmonary tuberculosis diagnosis and monitoring - 19/07/18

Doi : 10.1016/j.tube.2018.05.004

Yanfeng Zhao ^a,^b,^{^{1
These authors contributed equally to this work.}}, Xiqin Yang ^a,^{^{1
These authors contributed equally to this work.}}, Xuhui Zhang ^a,^{^{1
These authors contributed equally to this work.}}, Qin Yu ^c, Ping Zhao ^c, Jianxia Wang ^a, Cuimi Duan ^a, Jiangxue Li ^a, Heather Johnson ^d, Xiaoyan Feng ^a,^⁎ , Heqiu Zhang ^a,^⁎
^a Department of Bio-diagnosis, Institute of Basic Medical Sciences, 27, Taiping Road, Beijing, 100850, China
^b Beijing Research Institute for Tuberculosis Control, No 5, Dongguang Hutong, Xinjiekou, Beijing, 100035, China
^c Chaoyang District Center for Disease Control and Prevention, 25 Panjiayuan, Huaweili, Beijing, 100029, China
^d Olympia Diagnostics, Inc., Sunnyvale, CA 94086, USA

^∗Corresponding author. Department of Bio-diagnosis, Institute of Basic Medical Sciences, 27, Taiping Road, Beijing, 100850, China.Department of Bio-diagnosisInstitute of Basic Medical Sciences27, Taiping RoadBeijing100850China^∗∗Corresponding author.

Abstract

Objective

We aimed to determine whether IP-10 and RANTES plasma levels can be used in diagnosis and monitoring of pulmonary tuberculosis (PTB).

Methods

Plasma levels of cytokines/chemokines were measured using a Bio-Plex^® multiplex cytokine assay system in a cohort containing 457 clinically suspected PTB patients including a training set (n = 41)and two independent test sets A (n = 242) and B (n = 174).

Results

Plasma levels of IP-10 and RANTES were significantly higher in PTB patients than healthy controls' in both training and independent test sets (P < 0.05). Compared with other combinations, the combination of IP-10 and RANTES had the best performance with an AUC of 1.0 in training set. The performance characteristic of this model was successfully validated in independent test set A although this combination only resulted in a slightly improvement of AUC value in independent test set B. Plasma IP-10 and RANTES levels were weakly and positively correlated with blood glucose concentrations. Moreover, IP-10 levels were positively correlated with CRP and ESR in PTB patients. Furthermore, in response to therapy, both IP-10 and RANTES levels significantly decreased over the period of 6 months (P < 0.001).

Conclusions

Taken together, combination of IP-10 and RANTES could be potentially used as diagnostic and monitoring biomarker in PTB management.

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Keywords : Biomarkers, Pulmonary tuberculosis, Diagnosis, IP-10, RANTES

Plan

Study population characteristics

Identification of a two-cytokine signature in the training set

Validation of the two-cytokine signature in the independent test set A and B

Alteration of cytokines/chemokines levels following the anti-TB therapy

Discussion

Conflicts of interest

Author contributions

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Vol 111

P. 45-53 - juillet 2018 Retour au numéro

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IP-10 and RANTES as biomarkers for pulmonary tuberculosis diagnosis and monitoring - 19/07/18

Abstract

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Methods

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Plan

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