Evaluation of IP-10 in Quantiferon-Plus as biomarker for the diagnosis of latent tuberculosis infection - 19/07/18
, Valentina Vanini a , Teresa Chiacchio a , Elisa Petruccioli a , Gilda Cuzzi a , Vincenzo Schininà b , Fabrizio Palmieri c , Giuseppe Ippolito d , Delia Goletti a, ⁎ Abstract |
The QuantiFERON-TB Gold Plus (QFT-Plus) is a new test for latent tuberculosis infection (LTBI) diagnosis, in which has been added a new tube containing shorter peptides stimulating CD8 T-cells and CD4-stimulating-peptides. Measurement of alternative biomarkers to Interferon-γ (IFN-γ) in QFT-Plus may improve its sensitivity. Interferon-γ inducible protein 10 (IP-10), has been proposed as a tuberculosis (TB) biomarker. We aimed to evaluate the IP-10 accuracy in QFT-Plus for LTBI diagnosis.
QFT-Plus was performed in 36 active TB, 31 LTBI and 16 healthy donors (HD). IP-10 was detected by ELISA.
IP-10 is increased in TB1 and TB2 tubes in subjects with active TB and LTBI compared to HD. A ROC analysis comparing active TB and HD was performed and a cut-off of 1174 pg/mL for TB1 and 928.8 pg/mL for TB2 identified active TB with 86% sensitivity (Se) and 94% specificity (Sp). Moreover, increased IP-10 in response to TB1 was found in subjects with LTBI compared to those with active TB. A cut-off point of ≥16,108 pg/mL was chosen to maximize the test performance. However, the test predicted LTBI only with 58% Se and 61% Sp.
These results suggest that IP-10 is an alternative biomarker to IFN-γ in the QFT-Plus format.
Le texte complet de cet article est disponible en PDF.Keywords : Tuberculosis, IP-10, LTBI, Quantiferon-plus, Diagnosis, Inflammation
Plan
Vol 111
P. 147-153 - juillet 2018 Retour au numéro
Article précédent
- Characterization of pncA mutations in multi-drug and pyrazinamide resistant Mycobacterium tuberculosis isolates cultured from Queensland migrants and Papua New Guinea residents
- Sushil Pandey, Evelyn Lavu, Jacob Congdon, Rendi Moke, Arnold Bainomugisa, Christopher Coulter
- Detection of lipoarabinomannan in urine and serum of HIV-positive and HIV-negative TB suspects using an improved capture-enzyme linked immuno absorbent assay and gas chromatography/mass spectrometry
- Anita G. Amin, Prithwiraj De, John S. Spencer, Patrick J. Brennan, Joshua Daum, Barbara G. Andre, Maju Joe, Yu Bai, Lars Laurentius, Marc D. Porter, William J. Honnen, Alok Choudhary, Todd L. Lowary, Abraham Pinter, Delphi Chatterjee
Bienvenue sur EM-consulte, la référence des professionnels de santé.
L’accès au texte intégral de cet article nécessite un abonnement.
Déjà abonné à cette revue ?