The impact of repeated NALC/NaOH- decontamination on the performance of Xpert MTB/RIF assay - 18/05/18
, Elmar Saathoff a, b, Bariki Mtafya c, Daniel Mapamba c, Chacha Mangu c, Gabriel Rojas-Ponce c, Nyanda E. Ntinginya c, Martin Boeree d, Norbert Heinrich a, Stephen H. Gillespie e, ⁎ , Michael Hoelscher a, bon behalf of the
PanACEA-Consortium
Abstract |
The Xpert MTB/RIF assay detects Mycobacterium tuberculosis in unprocessed or NALC/NaOH- decontaminated sputum. The effect of repeated NALC/NaOH-decontamination on several Xpert performance parameters was assessed in this study. A second NALC/NaOH-decontamination had no effect on the binary Xpert-outcome but increased the value for the quantitative readout (CTmin). Repeated decontamination was not associated with PCR-inhibition or invalid results. The CTmin of M.tb positive samples was higher in inhibited Xpert runs. Our data indicate that NALC/NaOH-decontamination has an effect on the performance of the Xpert assay, and that CTmin readouts of decontaminated sputum samples should be interpreted with caution.
Le texte complet de cet article est disponible en PDF.Keywords : Xpert-inhibition, Sputum processing, Quantitative PCR, Xpert-performance
Plan
Vol 110
P. 56-58 - mai 2018 Retour au numéro
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