Genetic diversity of antigen 38?kDa in Mycobacterium tuberculosis strains from China - 18/05/18

Doi : 10.1016/j.tube.2018.03.003

Yingzhen Du ^a,^{^{1
These authors contributed equally to this work.}} , Hongxia Li ^a,^{^{1
These authors contributed equally to this work.}} , Xuebing Qin ^a,^{^{1
These authors contributed equally to this work.}} , Yan Wang ^b, Zhijian Zhang ^a, Geping Qu ^a, Xiangqun Fang ^a, Senyang Yu ^a,^⁎
^a Department of Respiratory, Nanlou Division, Chinese People's Liberation Army General Hospital, National Clinical Research Center for Geriatric Diseases, Beijing, China
^b Department of Emergency, Beijing Tsinghua Changgung Hospital, Tsinghua University, Changping District, Beijing, China

^∗Corresponding author.Department of Respiratory, Nanlou Division, Chinese People's Liberation Army General Hospital; National Clinical Research Center for Geriatric Diseases, 28# Fuxing Road, Haidian District, Beijing 100853, China.Department of RespiratoryNanlou DivisionChinese People's Liberation Army General HospitalNational Clinical Research Center for Geriatric Diseases28# Fuxing RoadHaidian DistrictBeijing100853China

Abstract

We used 335 Mycobacterium tuberculosis strains from 2010 National Epidemiologic Survey for TB in China and performed comparative sequence analysis of 38 kDa gene after amplification. From the results, we found that there were 5.07% M.tuberculosis strains that demonstrated genetic diversity of 38 kDa in China, and 2.99% strains showed polymorphism of the 38 kDa antigen, and this may be the reason for changes in the antigen produced, which may in turn cause alterations of related functions, thereby allowing immune evasion.

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Keywords : Genetic diversity, 38 kDa, Mycobacterium tuberculosis

Plan

Introduction

Materials and methods

Ethics statement

Strains and DNA preparation

PCR

Sequencing and data analysis

Spoligotyping

Statistical analysis

Results

Mutation and insertion

Spoligotyping and strain characteristic

Changes in T/B cell epitope of antigen 38 kDa

Discussion

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Vol 110

P. 20-25 - mai 2018 Retour au numéro

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Genetic diversity of antigen 38?kDa in Mycobacterium tuberculosis strains from China - 18/05/18

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