Integrating marker-assisted background analysis with foreground selection for pyramiding bacterial blight resistance genes into Basmati rice - 09/01/18
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Abstract |
Bacterial leaf blight (BB), caused by the bacterium Xanthomonas oryzae pv. Oryzae (Xoo), is the major constraint amongst rice diseases in India. CSR-30 is a very popular high-yielding, salt-tolerant Basmati variety widely grown in Haryana, India, but highly susceptible to BB. In the present study, we have successfully introgressed three BB resistance genes (Xa21, xa13 and xa5) from BB-resistant donor variety IRBB-60 into the BB-susceptible Basmati variety CSR-30 through marker-assisted selection (MAS) exercised with stringent phenotypic selection without compromising the Basmati traits. Background analysis using 131 polymorphic SSR markers revealed that recurrent parent genome (RPG) recovery ranged up to 97.1% among 15 BC3F1 three-gene-pyramided genotypes. Based on agronomic evaluation, BB reaction, aroma, percentage recovery of RPG, and grain quality evaluation, four genotypes, viz., IC-R28, IC-R68, IC-R32, and IC-R42, were found promising and advanced to BC3F2 generation.
Le texte complet de cet article est disponible en PDF.Keywords : Basmati rice, Bacterial leaf blight, Gene pyramiding, Molecular breeding, Marker assisted selection, Xanthomonas oryzae pv. oryzae
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Vol 341 - N° 1
P. 1-8 - janvier 2018 Retour au numéroBienvenue sur EM-consulte, la référence des professionnels de santé.
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