Ginkgolide B and bilobalide ameliorate neural cell apoptosis in α-synuclein aggregates - 15/12/17
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Highlights |
• | Recombinant α-Syn caused cytotoxicity to SH-SY5Y cell in a concentration-dependent manner. |
• | SH-SY5Y cell was more susceptible to α-Syn aggregates. |
• | α-Syn aggregates induce SH-SY5Y cell death in an apoptotic way. |
• | Ginkgolides attenuated cell apoptosis induced by α-Syn aggregates. |
Abstract |
The accumulation of aggregated forms of the α-Synuclein (α-Syn) is associated with the pathogenesis of Parkinson's disease (PD), a chronic progressive neurodegenerative disorder. Extensive evidences have shown the promising effects of Ginkgo biloba consumption on motor activity after PD. However, the mechanisms underline the α-Syn-induced cell damage and whether ginkgolides exert neuroprotection against this injury are unclear. Here we showed that aggregated recombinant human α-Syn, but not α-Syn monomers, triggered cell injury in cultured human neuroblastoma cell line SH-SY5Y in an apoptosis way by using flow cytometry and western blot assay. Moreover, pre-treatment with the Ginkgolide B (GB) or Bilobalide (BB) protected SH-SY5Y cells against α-Syn-induced cell viability decreases, and reduced cell apoptosis after aggregated α-Syn stimulation. Together, we firstly find that aggregated α-Syn induced cell apoptosis and GB and BB may attenuate aggregated α-Syn-induced cell apoptosis, which gives us an insight into the novel therapy for PD in future.
Le texte complet de cet article est disponible en PDF.Abbreviations : α-Syn, BB, GB, MPTP, MPP+, PD, SNpc
Keywords : α-Synuclein, Aggregates, Apoptosis, Ginkgolide B, Bilobalide
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Vol 96
P. 792-797 - décembre 2017 Retour au numéroBienvenue sur EM-consulte, la référence des professionnels de santé.
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