Enhanced neurogenesis in degenerated hippocampi following pretreatment with miR-302/367 expressing lentiviral vector in mice - 15/12/17
Bienvenue sur EM-consulte, la référence des professionnels de santé.
L’accès au texte intégral de cet article nécessite un abonnement.
| pages | 8 |
| Iconographies | 5 |
| Vidéos | 0 |
| Autres | 0 |
Abstract |
Astrogliosis is the main landmark of neurodegenerative diseases. In vivo reprogramming of reactive astrocytes to functional neurons opened a new horizon in regenerative medicine. However there is little evidence that show possible application of in vivo reprogramming approaches for enhancement of neurogenesis. Cluster miR-302/367 showed high capability in cell reprogramming. Here we show that application of lentiviral particles expressing cluster miR-302/367 along with systemic valproate (VPA) enhanced the capability of mice brains for neurogenesis in CA3 area following kainic acid (KA) induced hippocampal neurodegeneration. Following pretreatment with miR-302/367 expressing viral particles and VPA, transduced cells showed neuroblast and mature neuron markers when neuronal loss was induced by KA. Comparing the neuron counts in CA3 region also showed that neurogenesis was increased in CA3 region in animals which were pretreated with miR-302/367 vector and VPA, only in injected side of the brain. Our data suggest that targeted application of miR-302/367 expressing vector may enhance the capacity of hippocampus and other brain structures for regeneration following neuronal loss.
Le texte complet de cet article est disponible en PDF.Abbreviations : SVZ, SGZ, VPA, OPCs, KA, PBS, PFA, DAPI
Keywords : Astrogliosis, miR-302/367, In vivo, Reprogramming, Kainic acid, Hippocampus, Brain repair
Plan
Vol 96
P. 1222-1229 - décembre 2017 Retour au numéro
Article précédent
- LncRNA HOXD-AS1 promotes epithelial ovarian cancer cells proliferation and invasion by targeting miR-133a-3p and activating Wnt/β-catenin signaling pathway
- Ying Zhang, Yanbing Dun, Shumin Zhou, Xiang-Hua Huang
- Effects of standard ethanolic extract from Erythrina velutina in acute cerebral ischemia in mice
- Francisca Taciana Sousa Rodrigues, Caren Nádia Soares de Sousa, Naiara Coelho Ximenes, Anália Barbosa Almeida, Lucas Moraes Cabral, Cláudio Felipe Vasconcelos Patrocínio, Aline Holanda Silva, Luzia Kalyne Almeida Moreira Leal, José Eduardo Ribeiro Honório Júnior, Danielle Macedo, Silvânia Maria Mendes Vasconcelos
Bienvenue sur EM-consulte, la référence des professionnels de santé.
L’accès au texte intégral de cet article nécessite un abonnement.
Bienvenue sur EM-consulte, la référence des professionnels de santé.
L’achat d’article à l’unité est indisponible à l’heure actuelle.
Déjà abonné à cette revue ?