Multiple introductions and onward transmission of HIV-1 subtype B strains in Shanghai, China - 06/10/17

Doi : 10.1016/j.jinf.2017.05.009

Xiaoshan Li ^a,^{^{1
These authors contributed equally to this work.}}, Kexin Zhu ^b,^{^{1
These authors contributed equally to this work.}}, Yile Xue ^c,^{^{1
These authors contributed equally to this work.}}, Feiran Wei ^d, Rong Gao ^e, Ralf Duerr ^f, Kun Fang ^a, Wei Li ^g, Yue Song ^a, Guoping Du ^h, Wenjuan Yan ^a, Taha Hussein Musa ^a, You Ge ^a, Yu Ji ^a, Ping Zhong ^c,^⁎ , Pingmin Wei ^a,^⁎
^a Teaching and Research Office of Epidemiology and Health Statistics, School of Public Health, Southeast University, Nanjing, China
^b School of Public Health, Nantong University, Nantong, China
^c Department of AIDS and STD, Shanghai Municipal Center for Disease Control and Prevention, Shanghai Municipal Institutes for Preventive Medicine, Shanghai, China
^d Department of Oncology, Medical School of Southeast University, Nanjing, China
^e Department of Microbiology and Immunology, Medical School of Southeast University, Nanjing, China
^f Department of Pathology, New York University School of Medicine, New York, NY, USA
^g Department of Infectious Disease Prevention and School Health, Nanjing Municipal Center for Disease Control and Prevention, Nanjing, China
^h Hospital Office, Southeast University Hospital, Nanjing, China

^∗Corresponding author. Teaching and Research Office of Epidemiology and Health Statistics, School of Public Health, Southeast University, 87 Dingjiaqiao Road (W), Nanjing 210009, China. Fax: +86 25 83795531.Teaching and Research Office of Epidemiology and Health StatisticsSchool of Public HealthSoutheast University87 Dingjiaqiao Road (W)Nanjing210009China^∗∗Corresponding author. Department of AIDS and STD, Shanghai Municipal Center for Disease Control and Prevention, Shanghai Municipal Institutes for Preventive Medicine, 1380 Zhongshan Road (W), Shanghai 200336, China. Fax: +86 21 64338864.Department of AIDS and STDShanghai Municipal Center for Disease Control and PreventionShanghai Municipal Institutes for Preventive Medicine1380 Zhongshan Road (W)Shanghai200336China

Summary

Objective

To investigate the viral genetic evolution, spatial origins and patterns of transmission of HIV-1 subtype B in Shanghai, China.

Methods

A total of 242 Shanghai subtype B and 1519 reference pol sequences were subjected to phylogenetic inference and genetic transmission network analyses.

Results

Phylogenetic analysis revealed that subtype B strains circulating in Shanghai were genetically diverse and closely associated with viral sequence lineages in Beijing (76 of 242 [31.4%]), Central China (Henan/Hebei/Hunan/Hubei) (43 of 242 [17.8%]), Chinese Taiwan (20 of 242 [8.3%]), Japan (6 of 242 [2.5%]), and Korea (7 of 242 [2.9%]), suggesting multiple introductions into Shanghai from mainland China and Taiwan, Japan, and Korea. Interestingly, a monophyletic Shanghai lineage (SH-L) (36 of 242 [14.9%]) of HIV-1 subtype B most likely originated from an Argentine strain, transferred through Liaoning infected individuals. In-depth analyses of 195 Shanghai subtype B sequences revealed that a total of 37.9% (n = 74) sequences contributed to 35 transmission networks, whereof 33.8% (n = 25) of the sequences associated with infected individuals from other provinces.

Conclusions

Our new findings reflect the evolution complexity and transmission dynamics of HIV-1 subtype B in Shanghai, which would provide critical information for the design of effective prevention measures against HIV transmission.

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Highlights

•	HIV-1 subtype B genetic diversity in Shanghai was due to multiple introductions.
•	Japan and Korea subtype B have become the source of international dissemination.
•	HIV-1 subtype Thai-B strain has spread among the MSM population in Shanghai.
•	A unique Shanghai subtype B lineage most likely originated from Argentina.
•	Among 35 networks, one third of the Shanghai infections were linked to those in other provinces.

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Keywords : HIV-1, Subtype B, Phylogenetic analysis, Genetic transmission network analysis

Plan

Introduction

Methods

Sequence data

Phylogenetic analysis

Bayesian phylogeographic inference

Identification and analyses of genetic transmission networks

Ethics statement

Results

Presence of multiple subtype B transmission lineages that relate to other epidemic areas

The potential source and transmission route of the monophyletic Shanghai lineage (SH-L)

Evidence for multiple introductions of HIV-1 subtype B from Japan, Korea, and Taiwan into Shanghai

Transmission linkage of HIV-1 subtype B between Shanghai and other regions of China

Discussion

Limitations

Funding

Conflicts of interest

Author contributions

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Vol 75 - N° 2

P. 160-168 - août 2017 Retour au numéro

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Multiple introductions and onward transmission of HIV-1 subtype B strains in Shanghai, China - 06/10/17

Summary

Objective

Methods

Results

Conclusions

Highlights

Plan

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