Ameliorative effects of Artemisia pallens in a murine model of ovalbumin-induced allergic asthma via modulation of biochemical perturbations - 16/09/17
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Highlights |
• | Airway hyperresponsiveness (AHR) was induced in rats by ovalbumin (OVA). |
• | APME significantly attenuated OVA-induced alteration in lung functions. |
• | APME inhibited serum IgE, TNF-α, IL’s, TGF-β levels and decreased lung fibrosis. |
• | APME significantly inhibited oxido-nitrosative stress and increased lung Nrf2 level. |
• | Artemisia pallens exhibits its anti-asthmatic potential in OVA-induced AHR. |
Abstract |
Introduction |
Asthma is a chronic, heterogeneous airway disorder characterized by airway inflammatory and remodeling. Artemisia pallens has been reported to possess antioxidant, anti-inflammatory and Anti-allergic potential.
Objective |
To evaluate the anti-asthmatic effects of methanolic extract of Artemisia pallens (APME) against ovalbumin (OVA)-induced airway hyperresponsiveness (AHR) in rats.
Materials and method |
AHR was induced in male Sprague-Dawley rats (180–200g) by intraperitoneal (i.p.) injection of OVA and boosted with an identical OVA solution (s.c.) on day 7. Rats were either treated orally with vehicle (10mg/kg), montelukast (10mg/kg) or APME (100, 200 and 400mg/kg) for next 28days. At the end treatments, various biochemical, molecular (RT-PCR and ELISA analysis) and histological parameters were evaluated.
Results |
APME (200 and 400mg/kg) significantly attenuated (p<0.05) OVA-induced alteration in lung functions measured by Whole-body plethysmography. Increased Bronchoalveolar Lavage (BAL) fluid differential cell count, as well as total protein and albumin in BAL fluid and lungs, was significantly decreased (p<0.05) by APME. It also significantly attenuated (p<0.05) elevated lung oxido-nitrosative stress, myeloperoxidase, and serum IgE levels. OVA-induced down-regulation in lung Nrf2 and upregulation in TNF-α, IL-1β, IL-4, IL-6, TGF-β mRNA expression was significantly attenuated (p<0.05) by APME (200 and 400mg/kg) treatment. Histopathological analysis of lung tissue showed that APME treatment reduced OVA-induced inflammatory influx and fibrosis.
Conclusion |
Artemisia pallens simultaneously orchestrate plethora of mechanisms viz. modulations of IgE, TGF-β, TNF-α, IL’s and Nrf-2 levels to exhibit its anti-asthmatic potential in OVA-induced AHR in rats.
Le texte complet de cet article est disponible en PDF.Abbreviations : AHR, BPM, BALF, DBP, EIP, ELISA, Penh, EVE, f, GSH, IL’s, MDA, MABP, APME, MTL, MPO, NO, Nrf2, OVA, PEF, PIF, ROS, RT-PCR, SOD, SBP, TV, TGF-β, TNF-α
Keywords : Airway hyperresponsiveness, Artemisia pallens, Asthma, IgE, IL’s, Nrf-2, Ovalbumin, TGF-β, TNF-α
Plan
Vol 94
P. 880-889 - octobre 2017 Retour au numéro
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