Detection of Mycobacterium tuberculosis from paraffin-embedded tissues by GeneXpert MTB/RIF - 12/08/17
Doi : 10.1016/j.tube.2017.06.005
Laura Rindi a, ⁎ 
, Greta Alì b, Barbara Fabiani a, Gabriella Fontanini c, Carlo Garzelli a
, Greta Alì b, Barbara Fabiani a, Gabriella Fontanini c, Carlo Garzelli a a Department of Translational Research and New Technologies in Medicine and Surgery, University of Pisa, I-56127, Pisa, Italy
b Unit of Pathological Anatomy, University Hospital of Pisa, I-56126, Pisa, Italy
c Department of Surgical, Medical and Molecular Pathology and Critical Area, University of Pisa, I-56126, Pisa, Italy
∗Corresponding author. Dipartimento di Ricerca Traslazionale e delle Nuove Tecnologie in Medicina e Chirurgia, Via San Zeno, 35/39, 56127, Pisa, Italy.Dipartimento di Ricerca Traslazionale e delle Nuove Tecnologie in Medicina e ChirurgiaVia San Zeno, 35/39Pisa56127Italy
Abstract |
GeneXpert MTB/RIF (Xpert) assay, a rapid and automated system based on real-time PCR and molecular beacon technology, proved to be a sensitive and specific tool capable of detecting Mycobacterium tuberculosis and rifampin resistance in clinical specimens. In this study we provide a Xpert-dedicated successful protocol for processing paraffin-embedded tissue and assess the feasibility of the Xpert assay-based tuberculosis (TB) diagnosis on these specimens, thus proving the Xpert assay as a valuable TB diagnostic tool in supporting conventional histopathological methods.
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© 2017
Elsevier Ltd. Tous droits réservés.
Vol 106
P. 53-55 - septembre 2017 Retour au numéro
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