A yeast two-hybrid assay reveals CMYA1 interacting proteins - 02/08/17
, Ting Wang , Xinfeng Liu , Guoning Sui , Congfei Jin , Yingwei Yue , Shuping Yang , Hong Guo ⁎ Bienvenue sur EM-consulte, la référence des professionnels de santé.
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Abstract |
CMYA1 is a protein that plays an important role in muscle development and contains a highly conserved Xin repeats region. However, the function of the Xin-repeats in CMYA1 is unknown, and there is little information regarding proteins that interact with the CMYA1/Xin-repeats in the bovine system. In this study, we generated a high-quality bovine muscle cDNA library and performed a yeast two-hybrid screen twice using both CMYA1 and the Xin-repeats as bait. There were 27 candidate-interacting proteins identified in this screen. Three of the 27 proteins (RPL35A, RPL21 and EIF3G) interacted with both CMYA1 and the Xin-repeats, and this interaction was further confirmed using one-to-one Y2H mating. These results showed that the three candidate proteins interacted with CMYA1/Xin-repeats and indicated that the Xin-repeats is a key region of CMYA1 required for protein interaction. In conclusion, our results provide new targets on the bovine CMYA1/Xin-repeats interacting proteins, and these findings provide an important reference for the study of how bovine muscle development is regulated.
Le texte complet de cet article est disponible en PDF.Keywords : CMYA1, Yeast two-hybrid, Screen, Protein interaction, Muscle development
Abbreviations : CMYA1, RPL35A, RPL21, EIF3G, Y2H, PCR, cDNA, AD, BD, RT-PCR, A, X, DDO, QDO, YPDA
Plan
Vol 340 - N° 6-7
P. 314-323 - juin 2017 Retour au numéro
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