Knockdown of long noncoding RNA GHET1 inhibits cell activation of gastric cancer - 01/07/17
, Xueqiang Zhu a, Hao Liu a, Lijun Cai aBienvenue sur EM-consulte, la référence des professionnels de santé.
L’accès au texte intégral de cet article nécessite un abonnement.
| pages | 7 |
| Iconographies | 4 |
| Vidéos | 0 |
| Autres | 0 |
Abstract |
Objection |
The aim of this study was to evaluate the effects of lncRNA GHET1 in developing of Gastric Cancer.
Methods |
Collecting the 20 gastric cancer patients and evaluated the pathological of adjacent and tumor tissues by HE stating. We analyzed the protein expression of Numb in gastric cancer (GC) tissues by using IHC and the gene expression of Numb and GHET1 in adjacent and GC tissues by RT-PCR. The AGS cells were divided into 3 groups: Control (Co), NC and shRNA groups. Measuring the cell proliferation, apoptosis, cell cycle, invasion and migration abilities by MTT, flow cytometry, transwell and wound healing assays. We analyzed the relative signaling pathway by WB assay.
Results |
In the clinical analyzing, compared with adjacent tissues, the pathological was significantly changed in tumor tissues, the GHET1 gene and protein expressions were significantly increased in the GC tissues. In the cell experiment, down-regulation of GHET1 had suppressed the cell proliferation, invasion and migration activities and enhanced the cell apoptosis and G1 phase. We found that knockdown of GHET1 dramatically increased E-cadherin, while reducing fibronectin and vimentin.
Conclusion |
lncRNA GHET1 promoted AGS cells activations, and the results were shown that GHET1 dysregulation might be involved in the occurrence and development of gastric cancer. These results suggested that GHET1 might be a molecular marker for the progression of gastric cancer and a molecular target for targeted therapy.
Le texte complet de cet article est disponible en PDF.Keywords : Long noncoding RNAs (lncRNAs), Gastric cancer (GC), Gastric carcinoma highly expressed transcript 1 (GHET1), Proliferation, Apoptosis, Cell cycle, Invasion, Migration
Plan
Vol 92
P. 562-568 - août 2017 Retour au numéro
Article précédent
- Antibacterial, modulatory activity of antibiotics and toxicity from Rhinella jimi (Stevaux, 2002) (Anura: Bufonidae) glandular secretions
- Débora Lima Sales, Maria Flaviana Bezerra Morais-Braga, Antonia Thassya Lucas dos Santos, Antonio Judson Targino Machado, João Antonio de Araujo Filho, Diógenes de Queiroz Dias, Francisco Assis Bezerra da Cunha, Rogério de Aquino Saraiva, Irwin Rose Alencar de Menezes, Henrique Douglas Melo Coutinho, José Galberto Martins Costa, Felipe Silva Ferreira, Rômulo Romeu da Nóbrega Alves, Waltécio de Oliveira Almeida
- Hepatoprotective and antioxidant activity of quercetin loaded chitosan/alginate particles in vitro and in vivo in a model of paracetamol-induced toxicity
- Virginia Tzankova, Denitsa Aluani, Magdalena Kondeva-Burdina, Yordan Yordanov, Feodor Odzhakov, Alexandar Apostolov, Krassimira Yoncheva
Bienvenue sur EM-consulte, la référence des professionnels de santé.
L’accès au texte intégral de cet article nécessite un abonnement.
Bienvenue sur EM-consulte, la référence des professionnels de santé.
L’achat d’article à l’unité est indisponible à l’heure actuelle.
Déjà abonné à cette revue ?