Some proteins of M. tuberculosis that localise to the nucleus of THP-1-derived macrophages - 20/04/17
Summary |
Host-pathogen dialectics in tuberculosis (TB) via DNA-protein interactions are emerging. We investigated whether proteins produced by Mycobacterium tuberculosis (Mtb) could translocate to the host nucleus. Using lysates of nuclei purified from Mtb-infected THP-1-derived macrophages, we identified at least 15 proteins of Mtb-origin by electrophoretic and chromatographic separation and mass spectrometry. Western blotting confirmed time-dependent accumulation of Mtb EF-Tu, GroEL, GroES and MtrA in the host nucleus. MtrA could pull down at least 16 host proteins. Mtb proteins may have moonlighting functions that affect host gene expression.
Le texte complet de cet article est disponible en PDF.Keywords : Nuclear proteome, Nano-LC/MS, Protein–protein interactions, DNA-binding proteins, Moonlighting
Vol 101
P. 75-78 - décembre 2016 Retour au numéro
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