Mycobacterium smegmatis proteoliposome induce protection in a murine progressive pulmonary tuberculosis model - 20/04/17

Doi : 10.1016/j.tube.2016.07.017

Yanely Tirado ^a, Alina Puig ^a, Nadine Alvarez ^a, Reinier Borrero ^a, Alicia Aguilar ^a, Frank Camacho ^a, Fatima Reyes ^a, Sonsire Fernandez ^a, Jose Luis Perez ^a, Reynaldo Acevedo ^a, Dulce Mata Espinoza ^b, Jorge Alberto Barrios Payan ^b, Maria de los A. Garcia ^a, Ramlah Kadir ^c, María E. Sarmiento ^c, Rogelio Hernandez-Pando ^b, Mohd-Nor Norazmi ^c,^d,^⁎ , Armando Acosta ^d,^⁎
^a Institute Finlay, La Habana, Cuba
^b Experimental Pathology Section, Department of Pathology, National Institute of Medical Sciences and Nutrition “Salvador Zubiran”, D.F. Mexico, Mexico
^c School of Health Sciences, Universiti Sains Malaysia, Kelantan, Malaysia
^d INFORMM, Universiti Sains Malaysia, Kelantan, Malaysia

^∗Corresponding author.^∗∗Corresponding author. School of Health Sciences, Universiti Sains Malaysia, Kelantan, Malaysia.School of Health SciencesUniversiti Sains MalaysiaKelantanMalaysia

Summary

Tuberculosis (TB) remains an important cause of mortality and morbidity. The TB vaccine, BCG, is not fully protective against the adult form of the disease and is unable to prevent its transmission although it is still useful against severe childhood TB. Hence, the search for new vaccines is of great interest. In a previous study, we have shown that proteoliposomes obtained from Mycobacterium smegmatis (PLMs) induced cross reactive humoral and cellular response against Mycobacterium tuberculosis (Mtb) antigens. With the objective to evaluate the protective capability of PLMs, a murine model of progressive pulmonary TB was used. Animals immunized with PLMs with and without alum (PLMs/PLMsAL respectively) showed protection compared to non-immunized animals. Mice immunized with PLMsAL induced similar protection as that of BCG. Animals immunized with BCG, PLMs and PLMsAL showed a significant decrease in tissue damage (percentage of pneumonic area/lung) compared to non-immunized animals, with a more prominent effect in BCG vaccinated mice. The protective effect of the administration of PLMs in mice supports its future evaluation as experimental vaccine candidate against Mtb.

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Keywords : Mycobacterium smegmatis, Proteoliposomes, Tuberculosis, Vaccines, Challenge

Abbreviations : TB, Mtb, PL, BCG, CFU, PLMs, PLMsAL, OMV

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Vol 101

P. 44-48 - décembre 2016 Retour au numéro

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Mycobacterium smegmatis proteoliposome induce protection in a murine progressive pulmonary tuberculosis model - 20/04/17

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