Aberrant IgA responses to the gut microbiota during infancy precede asthma and allergy development - 19/04/17
, Maria C. Jenmalm, PhD a, ⁎∗ Abstract |
Background |
Although a reduced gut microbiota diversity and low mucosal total IgA levels in infancy have been associated with allergy development, IgA responses to the gut microbiota have not yet been studied.
Objective |
We sought to determine the proportions of IgA coating together with the characterization of the dominant bacteria, bound to IgA or not, in infant stool samples in relation to allergy development.
Methods |
A combination of flow cytometric cell sorting and deep sequencing of the 16S rDNA gene was used to characterize the bacterial recognition patterns by IgA in stool samples collected at 1 and 12 months of age from children staying healthy or having allergic symptoms up to 7 years of age.
Results |
The children with allergic manifestations, particularly asthma, during childhood had a lower proportion of IgA bound to fecal bacteria at 12 months of age compared with healthy children. These alterations cannot be attributed to differences in IgA levels or bacterial load between the 2 groups. Moreover, the bacterial targets of early IgA responses (including coating of the Bacteroides genus), as well as IgA recognition patterns, differed between healthy children and children with allergic manifestations. Altered IgA recognition patterns in children with allergy were observed already at 1 month of age, when the IgA antibodies are predominantly maternally derived in breast-fed children.
Conclusion |
An aberrant IgA responsiveness to the gut microbiota during infancy precedes asthma and allergy development, possibly indicating an impaired mucosal barrier function in allergic children.
Le texte complet de cet article est disponible en PDF.Key words : Allergic disease, asthma, secretory IgA, IgA index, IgA recognition patterns, microbiome composition, gut microbiota, childhood
Abbreviations used : ARC, FITC, IgA+, IgA−, LEfSe, qPCR, PCA, RDP, SIgA
Plan
| Supported by the Swedish Research Council (K2011-56X-21854-01-06); the Swedish Heart-Lung Foundation (20140321); the Ekhaga Foundation (210-53); the Medical Research Council of Southeast Sweden; the Olle Engqvist Foundation; the Cancer and Allergy Foundation; the University Hospital of Linköping, Sweden; and grant 2012-40007 from Spanish MINECO (to A.M.). |
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| Disclosure of potential conflict of interest: T. R. Abrahamsson has received grants and lecture fees from BioGaia. A. Mira has received grants from MINECO M. C. Jenmalm has received honorarium for lectures and Grants from BioGaia. The rest of the authors declare that they have no relevant conflicts of interest. |
Vol 139 - N° 3
P. 1017 - mars 2017 Retour au numéro
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