A deletion hampering appropriate typing of Mycobacterium africanum - 18/04/17

Doi : 10.1016/j.tube.2016.12.006

Estefanía Abascal ^a,^b, Diana Maricela Herrera ^a,^c, Marta Herranz ^a,^b,^d, Sheila Santantón ^a,^b, Miguel Martínez-Lirola ^e, Griselda Tudó ^f,^g, Juliá Gonzalez ^f,^g, Emilio Bouza ^a,^b,^d, Laura Pérez-Lago ^a,^b,^d,^⁎ , Darío García-de-Viedma ^a,^b,^d,^⁎
^a Servicio Microbiología Clínica y Enfermedades Infecciosas, Hospital General Universitario Gregorio Marañón, Madrid, Spain
^b Instituto de Investigación Sanitaria Gregorio Marañón, Madrid, Spain
^c Grupo de Investigación en Sanidad Animal y Humana, Departamento de Ciencias de la Vida y la Agricultura Universidad de las Fuerzas Armadas ESPE, Sangolquí, Ecuador
^d CIBER Enfermedades respiratorias, CIBERES, Spain
^e Servicio de Microbiología, Complejo Hospitalario Torrecárdenas, Almería, Spain
^f Servei de Microbiologia, Hospital Clinic-CDB, Barcelona, Spain
^g IS Global, Barcelona, Spain

^∗Corresponding authors. Servicio de Microbiología y Enfermedades Infecciosas, Hospital General Universitario Gregorio Marañón, Instituto de Investigación Sanitaria Gregorio Marañón, C/ Dr Esquerdo, 46, 28007 Madrid, Spain.Servicio de Microbiología y Enfermedades InfecciosasHospital General Universitario Gregorio MarañónInstituto de Investigación Sanitaria Gregorio MarañónC/ Dr Esquerdo, 46Madrid28007Spain

Abstract

Molecular epidemiology analysis of tuberculosis transmission is based mostly on the application of MIRU-VNTR. In certain isolates a complete 24-loci genotype is not obtained and these incompletely genotyped isolates can not be used in the definition of clusters. In a population-based molecular epidemiology study performed in Almería, Southeast Spain, a context with a high proportion of immigrants, we found that an 88-bp deletion in isolates of Mycobacterium africanum Lineage 5 hampers MIRU-VNTR analysis. A more extensive analysis revealed that this deletion was shared by all the Lineage 5 isolates in certain countries of origin of immigrants, such as Equatorial Guinea, and is likely present in several other African countries and also in the USA. A procedure is proposed to enable epidemiological analysis of these isolates.

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Keywords : Mycobacterium africanum, Genotyping, MIRU-VNTR, DNA deletion

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Vol 103

P. 24-27 - mars 2017 Retour au numéro

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A deletion hampering appropriate typing of Mycobacterium africanum - 18/04/17

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