Pulmonary immune responses against Aspergillus fumigatus are characterized by high frequencies of IL-17 producing T-cells - 18/04/17
, Renate de Boer a, e, Pleun Hombrink c, René E. Jonkers d, Jaap T. van Dissel b, J.H. Frederik Falkenburg a, Mirjam H.M. Heemskerk aSummary |
In healthy individuals and in patients with invasive aspergillosis, Aspergillus-specific T-cells in peripheral blood display mainly a Thelper1 phenotype. Although in other fungal infections Thelper17 immunity is important, it was suggested that in aspergillus infection Thelper17 cells do not play a role or may even be detrimental.
Objectives |
To compare the cytokine profiles of Aspergillus-specific CD4+ T-cells in peripheral blood and in the lung. To investigate the Thelper phenotype at the primary location of A. fumigatus exposure.
Methods |
Lung-derived T-cells and peripheral blood T-cells from COPD-patients were stimulated with overlapping peptides of 6 A. fumigatus proteins. Aspergillus-specific T-cells were identified on the basis of the activation marker CD154 and production of TNFα. In addition, production of the cytokines IFNγ, IL-17, IL-4 and IL-5 by the Aspergillus-specific T-cells was measured.
Results |
The majority of lung-derived Aspergillus-specific T-cells displayed a Thelper17 phenotype, and only low percentages of cells produced IFNγ. In contrast, in the peripheral blood of COPD patients Aspergillus-specific T-cells displayed a Thelper1 phenotype, similar as peripheral blood-derived Aspergillus-specific T-cells from healthy individuals.
Conclusions |
These data demonstrate that in A. fumigatus infection, similar as in other fungal infections, Thelper17 cells may play a more important role in the immune response than was appreciated until now.
Le texte complet de cet article est disponible en PDF.Highlights |
• | Frequencies of Aspergillus-specific T-cells in peripheral blood and lung are similar. |
• | Pulmonary Aspergillus-specific T-cells display a Thelper17 cytokine profile. |
• | Aspergillus-specific T-cells in peripheral blood have a Th1 phenotype. |
• | Activation and ability to produce cytokines are equal in both compartments. |
Keywords : Aspergillus fumigatus, Lung diseases, fungal, T-lymphocytes, Th17 cells, Cytokines
Plan
Vol 74 - N° 1
P. 81-88 - janvier 2017 Retour au numéro
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