Visualizing viable Mycobacterium tuberculosis in sputum to monitor isolation measures - 18/04/17
Doi : 10.1016/j.jinf.2016.11.014
Benjamin Wyplosz ⁎ 
AP-HP, CHU de Bicêtre, Service de maladies infectieuses et tropicales, Le Kremlin-Bicêtre, France
∗Corresponding author. Fax: +33 1 45 21 74 23.
Faiza Mougari
AP-HP, Hôpital Lariboisière, Laboratory of Bacteriology, Paris, France
Université Paris Diderot, INSERM IAME UMR1137, Sorbonne Paris Cité, Paris, France
National Reference Center for Mycobacteria and Antimycobacterial Resistance, Paris, France
May Al Rawi
AP-HP, Hôpital Lariboisière, Laboratory of Bacteriology, Paris, France
National Reference Center for Mycobacteria and Antimycobacterial Resistance, Paris, France
Céline Baillon
Centre Médico-chirugical de Bligny, Laboratoire de bactériologie, Briis-sous-Forges, France
Dhiba Marigot-Outtandy, Damien Le Dû, Mathilde Jachym
Centre Médico-chirugical de Bligny, Sanatorium, Briis-sous-Forges, France
Vincent Hervé
Centre Médico-chirugical de Bligny, Laboratoire de bactériologie, Briis-sous-Forges, France
Laurent Raskine
AP-HP, Hôpital Lariboisière, Laboratory of Bacteriology, Paris, France
National Reference Center for Mycobacteria and Antimycobacterial Resistance, Paris, France
Emmanuelle Cambau
AP-HP, Hôpital Lariboisière, Laboratory of Bacteriology, Paris, France
Université Paris Diderot, INSERM IAME UMR1137, Sorbonne Paris Cité, Paris, France
National Reference Center for Mycobacteria and Antimycobacterial Resistance, Paris, France
Highlights |
• | Discontinuing isolation for tuberculosis patients under treatment relies on the infectiousness of respiratory specimens. |
• | Assessing the viability of Mycobacterium tuberculosis in sputum takes between 10 and 42 days in liquid media. |
• | We adapted a commercialized kit (Live/Dead® BacLight™) to monitor the viability of bacilli in the sputa of 18 TB patients. |
• | The viability test predicted accurately (specificity 100%), rapidly (<1 h) and at a low cost (<1 €) the result of cultures. |
• | The viability test could help worldwide in the decision to maintain isolation measures. |
Keywords : Tuberculosis, Isolation, Hospital infection control, Viability stain, Treatment response
Plan
© 2016
The British Infection Association. Publié par Elsevier Masson SAS. Tous droits réservés.
Vol 74 - N° 2
P. 207-210 - février 2017 Retour au numéro
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