Comparison of the DWI and Gd-EOB-DTPA-enhanced MRI on assessing the hepatic ischemia and reperfusion injury after partial hepatectomy - 17/01/17
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Abstract |
Objective |
To compare two different imaging media, diffusion weighted imaging (DWI) with apparent diffusion coefficient (ADC) and Gd-EOB-DTPA-enhanced magnetic resonance imaging (MRI) with perfusion parameters Ktrans, Kep, and relative contrast enhancement index (RCEI), in assessing the liver function via ischemia/perfusion injury (IRI) + partial hepatectomy rat model.
Methods |
Rats underwent 0, 30 and 60min of ischemia/reperfusion with 30% of hepatectomy before subjected to Gd-EOB-DTPA-enhanced MRI in addition to 99mTc-GSA scintigraphy. For 99mTc-GSA scintigraphy test, the receptor index LHL15, modified receptor index and the blood clearance index HH15 were recorded. Apparent diffusion coefficient (ADC) was evaluated by using both mono- and bi-exponential models, and perfusion parameters Ktrans, Kep, and RCEI were measured. Liver function is tested by measuring activity of serum ALT, AST and PT. Histological analysis was performed by H&E and Ki-67 staining.
Results |
99mTc-GSA dynamic imaging analysis demonstrated that LHL15 was increased and HH15 was decreased as the extension of ischemia/reperfusion time. ADC value estimated by MRI was significantly increased (P<0.05) in 30min IRI group compared with 0min and 60min IRI groups, respectively. Ktrans value was gradually and significantly decreased (P<0.05) as the extension of IRI time, but there was no significant difference (P>0.05) in Kep value between at 30min and 60min IRI, and RCEI value was significantly higher (P<0.05) in 30min IR compared with 0min and 60min IRI group. Serum level of ALT, AST and PT were gradually and significantly (P<0.05) increased as the extension of IRI time. Histological analysis showed that there was a remarkable difference between 30min and 60min IRI, as protein expression of Ki-67 was significantly higher (P<0.05) in 30min IRI group.
Conclusion |
Fast ADC bi-exponential model in DWI and RCEI in Gd-EOB-DTPA-enhanced MRI showed the good correlation in assessment of liver function after partial hepatectomy, showing consistency with our histological findings. The Ktrans in Gd-EOB-DTPA-enhanced MRI could be a potent parameter for assessing the early ischemic injury, but not the severity of the hepatic injury, in accordance with the correlation with our biochemical findings.
Le texte complet de cet article est disponible en PDF.Keywords : Magnetic resonance imaging, Ischemia/reperfusion injury, Liver
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Vol 86
P. 118-126 - février 2017 Retour au numéroBienvenue sur EM-consulte, la référence des professionnels de santé.
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