Transcription factors regulating uspA genes in Catharanthus roseus - 11/01/17

Doi : 10.1016/j.crvi.2016.10.004

Ahmed Bahieldin ^a,^b,^⁎ , Ahmed Atef ^a , Ahmed M. Shokry ^a,^c , Saleh Al-Karim ^a , Sanaa G. Al Attas ^a , Nour O. Gadallah ^a,^d , Sherif Edris ^a,^b,^e , Magdy A. Al-Kordy ^a,^d, Sabah M. Hassan ^a,^b , Salah Abo-Aba ^a,^f , Fotouh M. El-Domyati ^a,^b
^a Department of Biological Sciences, Faculty of Science, King Abdulaziz University (KAU), P.O. Box 80141, 21589 Jeddah, Saudi Arabia
^b Department of Genetics, Faculty of Agriculture, Ain Shams University, Cairo, Egypt
^c Agricultural Genetic Engineering Research Institute (AGERI), Agriculture Research Center (ARC), Giza, Egypt
^d Genetics and Cytology Department, Genetic Engineering and Biotechnology Division, National Research Center, Dokki, Egypt
^e Princess Al-Jawhara Al-Brahim Centre of Excellence in Research of Hereditary Disorders (PACER-HD), Faculty of Medicine, King Abdulaziz University (KAU), Jeddah, Saudi Arabia
^f Microbial Genetics Department, Genetic Engineering and Biotechnology Division, National Research Centre, Giza, Egypt

^⁎Corresponding author. Department of Biological Sciences, Faculty of Science, King Abdulaziz University (KAU), P.O. Box 80141, Jeddah 21589, Saudi Arabia.

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Abstract

RNA-Seq of the Catharanthus roseus SRA database was done in order to detect putative universal stress proteins (USPs) and their possible controlling factors. Previous analysis indicated the existence and characterization of uspA-like genes. In silico analysis of RNA-Seq database in several plant tissues revealed the possible functions and regulations of some uspA-like transcripts whose transcription factors (TFs) that might drive their expression were detected. BLAST indicated the existence of TF superfamilies erf (ethylene-responsive TF), bHLH (basic helix-loop-helix) and WRKY that might regulate several uspA-like genes. This data was proven via semi-quantitative RT-PCR in four plant tissues. Several of these transcription factor superfamilies are known for their action in the plant defense against biotic and abiotic stresses.

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Keywords : Transcription factor, Co-expression, Erf, BHLH, WRKY, RNA-Seq, SqRT-PCR

Abbreviations : USP, TF, bHLH, ERF, sqRT-PCR, NCBI, TIA, DE, iML, ML, FL, R, MTI, PTI, ETI, Trp, JA, SA

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Vol 340 - N° 1

P. 1-6 - janvier 2017 Retour au numéro

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Transcription factors regulating uspA genes in Catharanthus roseus - 11/01/17

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