The efficacy of aged garlic extract (AGE) in alleviating the hepatic inflammation and pulmonary fibrotic responses induced by titanium dioxide (TiO₂) toxicity was studied. The control group received distilled water. AGE-treated group received AGE (2ml/kgbw). TiO₂- intoxicated group received TiO₂ (5g/kgbw). AGE combined with TiO₂ treated group administered AGE prior TiO₂ by six hours at the same previously doses. All treatments were given orally every other day for two months. TiO_2- intoxicated rats elicited a significant up-regulation in hepatic TLR-2, TLR-4, NF-?B/p65, CYP1B1 and CYP2B1 with pulmonary MMP-9, TIMP-1, TGF-ß1, collagen-1a, fibronectin mRNA. Moreover, a significant elevation in serum AST, ALT, LDH, ß-glucuronidase, N-acetyl ß glucosaminidase, hydroxyproline, MMP-9, TIMP-1, fibronectin, TGF-ß1 levels with a significant decrease in total protein that ensued from TiO₂ toxicity. The ultra-histopathological findings reinforced these results. The co-administration of AGE and TiO₂ alleviated these changes. These data support the nutraceutical role of AGE against TiO₂ toxicity.