The impact of methotrexate on lung inflammatory and apoptotic pathway biomarkers—The role of gallic acid - 03/01/17
, Onder Ozturk b, Ozlem Ozmen c, Ilter Ilhan d, Taner Gonca e, Nurhan Gumral a, Hikmet Orhan f, Rahime Aslankoc aBienvenue sur EM-consulte, la référence des professionnels de santé.
L’accès au texte intégral de cet article nécessite un abonnement.
| pages | 8 |
| Iconographies | 8 |
| Vidéos | 0 |
| Autres | 0 |
Abstract |
Backgrounds |
The aim of this study was to investigate the effects of methotrexate (MTX) on the lung via inflammatory and apoptotic pathway biomarkers and the role of gallic acid (GA).
Methods |
In this study, twenty four male Wistar-Albino rats weighing 300–350g were divided into 3 groups as follows; Control group (0.1ml/oral saline, for 7 days+2nd day i.p.). MTX group (20mg/kg, single dose, on 2nd day). MTX+GA group (15mg/kg, orally, for 7 days). Comet analysis, oxidant-antioxidant status, IMA were conducted. Histopathological analyses were evaluated.
Results |
Comet assay on the blood, TOS and OSI values in the lung were increased in the group II compared with the control group (p<0.05). GA significantly reduced the comet score and IMA levels in the blood, TOS and OSI values in the lung tissue in group III compared with group II (p<0.05). Immunohistochemically PGE2, TNF-α, CRP, serum SAA, Caspase 3 and Caspase 9 expressions significantly increased in group II compared with the control group (p<0.001) and GA treatment ameliorated these parameters significantly in group III compared with group II (p<0.001).
Conclusions |
MTX caused oxidative stress and DNA damage in the blood tissue and caused oxidative damage, inflammation and apoptosis in the lung tissue.
Le texte complet de cet article est disponible en PDF.Keywords : Lung, Methotrexate, Gallic acid, Oxidative stress, Inflammation
Plan
Vol 84
P. 1689-1696 - décembre 2016 Retour au numéro
Article précédent
- Metformin preconditioned adipose derived mesenchymal stem cells is a better option for the reversal of diabetes upon transplantation
- Nitya Shree, Ramesh R Bhonde
- Effect of patchouli (Pogostemon cablin) essential oil on in vitro and in vivo leukocytes behavior in acute inflammatory response
- Saulo Euclides Silva-Filho, Luiz Alexandre Marques Wiirzler, Heitor Augusto Otaviano Cavalcante, Nancy Sayuri Uchida, Francielli Maria de Souza Silva-Comar, Gabriel Fernando Esteves Cardia, Expedito Leite da Silva, Rafael Pazinatto Aguiar, Ciomar Aparecida Bersani-Amado, Roberto Kenji Nakamura Cuman
Bienvenue sur EM-consulte, la référence des professionnels de santé.
L’accès au texte intégral de cet article nécessite un abonnement.
Bienvenue sur EM-consulte, la référence des professionnels de santé.
L’achat d’article à l’unité est indisponible à l’heure actuelle.
Déjà abonné à cette revue ?