Gallic acid reduces cell growth by induction of apoptosis and reduction of IL-8 in HepG2 cells - 03/01/17
, Gabriele Catyana Krause a, Aline Daniele Schuster b, Anderson Velasque Catarina c, Bruno Souza Basso a, Fernanda Cristina De Mesquita a, Leonardo Pedrazza a, Elisa Simon Marczak d, Bianca Andrade Martha a, Fernanda Bordignon Nunes a, Eduardo Cremonese Filippi Chiela e, Natália Jaeger d, Marcos Paulo Thomé f, Gabriela Viegas Haute a, Henrique Bregolin Dias a, Márcio Vinícius Fagundes Donadio g, Jarbas Rodrigues De Oliveira aBienvenue sur EM-consulte, la référence des professionnels de santé.
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Abstract |
Hepatocellular carcinoma is the most prevalent primary liver tumor and is among the top ten cancer that affect the world population. Its development is related, in most cases, to the existence of chronic liver injury, such as in cirrhosis. The knowledge about the correlation between chronic inflammation and cancer has driven new researches with anti-inflammatory agents that have potential for the development of antitumor drugs. Gallic acid is a phenolic acid found in many natural products and have shown anti-inflammatory, anti-tumor, anti-mutagenic and antioxidant actions. The purpose of this study was to investigate the effect of gallic acid on acute and chronic cell proliferation and inflammatory parameters of hepatocellular carcinoma cells (HepG2), as well as to investigate the mechanisms involved. Results showed that the gallic acid decreased the proliferation of HepG2 cells in a dose-dependent manner (Trypan blue exclusion assay), without causing necrosis (LDH assay). We observed a significant increase in the percentage of small and regular nuclei (Nuclear Morphometric Analysis assay), a significant induction of apoptosis by Annexin V-FITC and PI assay and no interference with the cell cycle using the FITC BrdU Flow Kit. We observed a significant reduction in the levels of IL-8 and increased levels of IL-10 and IL-12 (Cytometric Bead Array Human Inflammation Assay). Furthermore, gallic acid caused no cancer cells regrowth at a long term (Cumulative Population Doubling assay). According to these results, gallic acid showed a strong potential as an anti-tumor agent in hepatocellular carcinoma cells.
Le texte complet de cet article est disponible en PDF.Abbreviations : 7-AAD, ANOVA, BrdU, CC50, CPD, DAPI, DMEM, FBS, FITC, GA, HCC, HEPES, HepG2, IL-10, IL-12, IL-1β, IL-6, IL-8, LDH, N(t), N(to), PBS, PD, PI, SEM, Triton X-100
Keywords : Gallic acid, HepG2 cells, Apoptosis, Interleukin-8, Interleukin-10, Interleukin-12
Plan
Vol 84
P. 1282-1290 - décembre 2016 Retour au numéro
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